Phospho-PKA R2 (S99) Rabbit Recombinant mAb Datasheet

生物描述

特异性	Phospho-PKA R2 (S99) Rabbit Recombinant mAb detects endogenous level of total Phospho-PKA R2 (S99).
背景	Intracellular effects of cAMP are conveyed by cyclic AMP-dependent protein kinase (PKA) types I and II in eukaryotic cells. The PKA type II holoenzyme consists of two catalytic subunits and two regulatory subunits (RIIα or RIIβ) that bind to and inactivate the catalytic subunits. PKA is activated by the binding of two cAMP molecules to each R subunit, which promotes release of the catalytic subunits from the R-cAMP complex. Active catalytic subunits phosphorylate specific substrates and can be translocated to the nucleus, where they activate cAMP-responsive genes. The subcellular localization and activity of protein kinases is often altered by protein phosphorylation. RII can be autophosphorylated by the catalytic subunit of PKA. Human RIIα is autophosphorylated on S99 throughout the cell cycle, and is hyperphosphorylated on T54 at mitosis and by purified CDK1 in vitro.

特异性

Phospho-PKA R2 (S99) Rabbit Recombinant mAb detects endogenous level of total Phospho-PKA R2 (S99).

背景

Intracellular effects of cAMP are conveyed by cyclic AMP-dependent protein kinase (PKA) types I and II in eukaryotic cells. The PKA type II holoenzyme consists of two catalytic subunits and two regulatory subunits (RIIα or RIIβ) that bind to and inactivate the catalytic subunits. PKA is activated by the binding of two cAMP molecules to each R subunit, which promotes release of the catalytic subunits from the R-cAMP complex. Active catalytic subunits phosphorylate specific substrates and can be translocated to the nucleus, where they activate cAMP-responsive genes. The subcellular localization and activity of protein kinases is often altered by protein phosphorylation. RII can be autophosphorylated by the catalytic subunit of PKA. Human RIIα is autophosphorylated on S99 throughout the cell cycle, and is hyperphosphorylated on T54 at mitosis and by purified CDK1 in vitro.

使用信息

抗体应用

WB ,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat Pig

MW (kDa)

51kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Landsverk HB, et al. J Cell Sci. 2001, 114(Pt 18):3255-64.

Application Data

WB

Validated by Selleck