AMPK beta 1 Rabbit Recombinant mAb Datasheet

生物描述

特异性	AMPK beta 1 Rabbit Recombinant mAb detects endogenous level of total AMPK beta 1.
背景	AMPK is a heterotrimeric protein consisting of one catalytic subunit (α) and two non-catalytic subunits (β and γ). Two isoforms of α and β subunits (α1 and α2, and β1 and β2), and three isoforms of the γ subunit (γ1, γ2 and γ3) have been identified. AMPK is a primary sensor of cellular energy changes. In response to environmental stresses that cause ATP depletion in cells, the kinase activity of AMPK is instantly induced and the increased activity either inhibits ATP consuming pathways or promotes ATP producing pathways. Three mechanisms were identified that contribute to the activation of the kinase: (i) a direct allosteric activation; (ii) activation through phosphorylation by an upstream kinase and (iii) maintaining the activity through inhibition of dephosphorylation. The β1 subunit of AMPK binds to two other subunits, a and g in a 1:1:1 ratio and serves as a ‘bridge' between them to form a stable trimeric complex. β subunit might modulate the kinase activity through regulation of cellular localization of the enzyme. The β1 subunit can be transcripitionally upregulated by cold shock and a DNA damaging agent, etoposide, and such induction is independent of p53. And forced expression of AMPK-β1 inhibits tumor cell growth.

特异性

AMPK beta 1 Rabbit Recombinant mAb detects endogenous level of total AMPK beta 1.

背景

AMPK is a heterotrimeric protein consisting of one catalytic subunit (α) and two non-catalytic subunits (β and γ). Two isoforms of α and β subunits (α1 and α2, and β1 and β2), and three isoforms of the γ subunit (γ1, γ2 and γ3) have been identified. AMPK is a primary sensor of cellular energy changes. In response to environmental stresses that cause ATP depletion in cells, the kinase activity of AMPK is instantly induced and the increased activity either inhibits ATP consuming pathways or promotes ATP producing pathways. Three mechanisms were identified that contribute to the activation of the kinase: (i) a direct allosteric activation; (ii) activation through phosphorylation by an upstream kinase and (iii) maintaining the activity through inhibition of dephosphorylation. The β1 subunit of AMPK binds to two other subunits, a and g in a 1:1:1 ratio and serves as a ‘bridge' between them to form a stable trimeric complex. β subunit might modulate the kinase activity through regulation of cellular localization of the enzyme. The β1 subunit can be transcripitionally upregulated by cold shock and a DNA damaging agent, etoposide, and such induction is independent of p53. And forced expression of AMPK-β1 inhibits tumor cell growth.

使用信息

抗体应用

WB, IF,ELISA,ELISA

稀释比例

WB	IF
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

38kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Li J, et al. Carcinogenesis. 2003, 24(5):827-34.

Application Data

WB

Validated by Selleck

IF

Validated by Selleck