APC Rabbit Recombinant mAb Datasheet

生物描述

特异性	APC Rabbit Recombinant mAb detects endogenous level of total APC.
背景	The adenomatous polyposis coli (APC) protein is a multi-domain protein that contains binding sites for numerous proteins, including microtubules, the Wnt/Wg pathway components β-catenin and axin, the cytoskeletal regulators EB1 and IQGAP1, and the Rac guanine-nucleotide-exchange factor (GEF) Asef1. It is atumor suppressor involved in several cellular processes and signal transduction pathways such as the canonical Wnt signal transcription, cell adhesion, cell migration and chromosomal segregation. APC plays a central role suppressing the canonical Wnt signalling pathway that controls cell proliferation and differentiation in various organs. APC inhibits β-catenin/TCF-dependent transcription through multiple mechanisms. It could provide a scaffold for a destruction complex together with GSK3β and axin that promotes phosphorylation and consequent ubiquitin-dependent degradation of β-catenin. Second, APC promotes export of β-catenin from the nucleus, which reduces the amount of nuclear β-catenin/TCF. Third, APC binds to β-catenin, blocking the interaction with TCF. Finally, recent studies show that APC can inhibit β-catenin/TCF-dependent transcription through a direct interaction with a repressor complex.

特异性

APC Rabbit Recombinant mAb detects endogenous level of total APC.

背景

The adenomatous polyposis coli (APC) protein is a multi-domain protein that contains binding sites for numerous proteins, including microtubules, the Wnt/Wg pathway components β-catenin and axin, the cytoskeletal regulators EB1 and IQGAP1, and the Rac guanine-nucleotide-exchange factor (GEF) Asef1. It is atumor suppressor involved in several cellular processes and signal transduction pathways such as the canonical Wnt signal transcription, cell adhesion, cell migration and chromosomal segregation. APC plays a central role suppressing the canonical Wnt signalling pathway that controls cell proliferation and differentiation in various organs. APC inhibits β-catenin/TCF-dependent transcription through multiple mechanisms. It could provide a scaffold for a destruction complex together with GSK3β and axin that promotes phosphorylation and consequent ubiquitin-dependent degradation of β-catenin. Second, APC promotes export of β-catenin from the nucleus, which reduces the amount of nuclear β-catenin/TCF. Third, APC binds to β-catenin, blocking the interaction with TCF. Finally, recent studies show that APC can inhibit β-catenin/TCF-dependent transcription through a direct interaction with a repressor complex.

使用信息

抗体应用

WB, IF,ELISA,ELISA

稀释比例

WB	IF
1:500	1:50

反应性

Human Rat

MW (kDa)

160kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Aoki K, et al. J Cell Sci. 2007, 120(Pt 19):3327-35.

Application Data

WB

Validated by Selleck

IF

Validated by Selleck