Aurora B Rabbit Recombinant mAb

生物描述

特异性

Aurora B Rabbit Recombinant mAb detects endogenous levels of total Aurora B.

背景

Aurora B, a member of the Aurora family of serine/threonine protein kinases, is a key player in chromosome segregation. As part of a macromolecular complex known as the chromosome passenger complex, Aurora B concentrates early during mitosis in the proximity of centromeres and kinetochores, the sites of attachment of chromosomes to spindle microtubules. There, it contributes to a number of processes that impart fidelity to cell division, including kinetochore stabilization, kinetochore-microtubule attachment, and the regulation of a surveillance mechanism named the spindle assembly checkpoint. In the regulation of these processes, Aurora B is the fulcrum of a remarkably complex network of interactions that feed back on its localization and activation state. Aurora B, part of the chromosome passenger complex (CPC), is located on the chromosome arms during prophase and at the centromeres during prometaphase and metaphase. Aurora B subsequently localizes to the midbody duringcytokinesis. Aurora B Interacts with p53 During both Interphase and Mitosis, phosphorylates p53 and enhances p53 ubiquitination. Thus, Aurora B negatively regulates p53.

使用信息

抗体应用	WB, IF,ELISA,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human
MW (kDa)	39kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Krenn V, et al. Front Oncol. 2015, 5:225. [2] Gully CP, et al. Proc Natl Acad Sci U S A. 2012, 109(24):E1513-22.

Application Data

WB

IF