Bid Rabbit Recombinant mAb Datasheet

生物描述

特异性	Bid Rabbit Recombinant mAb detects endogenous level of total Bid.
背景	Bid, an acronym for BH3-interacting domain death agonist, is a member of the Bcl-2 family of proteins that regulate the permeabilization of the outer mitochondrial membrane (OMM), a critical event during apoptosis. It is a pro-apoptotic member. During apoptosis, Bid can be cleaved not only by caspase-8 during death receptor apoptotic signaling, but also by other caspases, granzyme B, calpains and cathepsins. Protease-cleaved Bid migrates to mitochondria where it induces permeabilization of the outer mitochondrial membrane that is dependent on the pro-apoptotic proteins Bax and/or Bak, and thus Bid acts as a sentinel for protease-mediated death signals.

特异性

Bid Rabbit Recombinant mAb detects endogenous level of total Bid.

背景

Bid, an acronym for BH3-interacting domain death agonist, is a member of the Bcl-2 family of proteins that regulate the permeabilization of the outer mitochondrial membrane (OMM), a critical event during apoptosis. It is a pro-apoptotic member. During apoptosis, Bid can be cleaved not only by caspase-8 during death receptor apoptotic signaling, but also by other caspases, granzyme B, calpains and cathepsins. Protease-cleaved Bid migrates to mitochondria where it induces permeabilization of the outer mitochondrial membrane that is dependent on the pro-apoptotic proteins Bax and/or Bak, and thus Bid acts as a sentinel for protease-mediated death signals.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human

MW (kDa)

22kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Billen LP, et al. Oncogene. 2008, 27 Suppl 1:S93-104.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck