Cathepsin L/V/K/H Rabbit Recombinant mAb Datasheet

生物描述

特异性	Cathepsin L/V/K/H Rabbit Recombinant mAb detects endogenous level of total Cathepsin L/V/K/H.
背景	There are 11 human cysteine cathepsins, i.e., the cathepsins B, C, F, H, K, L, O, S, V, X and W. The majority of cathepsins are ubiquitously expressed in human tissues such as cathepsins B, H, L, C, X, F, O and V. Their expression profile indicates that these enzymes are involved in a normal cellular protein degradation and turnover. In contrast, cathepsins K, W and S show a restricted cell or tissue-specific distribution. Cathepsin K is highly expressed in osteoclasts, in most epithelial cells and in the synovial fibroblasts in rheumatoid arthritis joints. Cathepsin V (also named L2) is highly homologous to cathepsin L, but in contrast to the ubiquitously expressed cathepsin L, its expression is restricted to thymus and testis. Active cathepsins are also localized in other cellular compartments, such as the nucleus, cytoplasm and plasma membrane. Cysteine cathepsins exhibit broad specificity, thus cleaving their substrates preferentially after basic or hydrophobic residues. They are believed to participate exclusively in terminal protein degradation during necrotic and autophagic cell death. They also execute numerous specific functions participating in important physiological processes, such as MHC-II-mediated antigen presentation, bone remodeling, keratinocyte differentiation, prohormones activation, and others.

特异性

Cathepsin L/V/K/H Rabbit Recombinant mAb detects endogenous level of total Cathepsin L/V/K/H.

背景

There are 11 human cysteine cathepsins, i.e., the cathepsins B, C, F, H, K, L, O, S, V, X and W. The majority of cathepsins are ubiquitously expressed in human tissues such as cathepsins B, H, L, C, X, F, O and V. Their expression profile indicates that these enzymes are involved in a normal cellular protein degradation and turnover. In contrast, cathepsins K, W and S show a restricted cell or tissue-specific distribution. Cathepsin K is highly expressed in osteoclasts, in most epithelial cells and in the synovial fibroblasts in rheumatoid arthritis joints. Cathepsin V (also named L2) is highly homologous to cathepsin L, but in contrast to the ubiquitously expressed cathepsin L, its expression is restricted to thymus and testis. Active cathepsins are also localized in other cellular compartments, such as the nucleus, cytoplasm and plasma membrane. Cysteine cathepsins exhibit broad specificity, thus cleaving their substrates preferentially after basic or hydrophobic residues. They are believed to participate exclusively in terminal protein degradation during necrotic and autophagic cell death. They also execute numerous specific functions participating in important physiological processes, such as MHC-II-mediated antigen presentation, bone remodeling, keratinocyte differentiation, prohormones activation, and others.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

38kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Olson OC, et al. Nat Rev Cancer. 2015, 15(12):712-29.

Application Data

WB

Validated by Selleck