生物描述

特异性

CD3D Rabbit Recombinant mAb detects endogenous levels of total CD3D.

背景

CD3 delta (CD3D) is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) that plays essential roles in adaptive immune response and is involved in T-cell development and signal transduction. CD3 complex is a common surface marker on T cells. It has important functions not only as an essential component in forming the T cell receptor (TCR)-CD3 complex, but also as an external signal transducer. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human
MW (kDa)	19kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Otoya Ueda, et al. Scientific Reports. 2017, 7, Article number: 45839. [2] Barber EK, et al. Proc Natl Acad Sci U S A. 1989, 86(9):3277-81. [3] Doucey MA, et al. J Biol Chem. 2003, 278(5):3257-64.

Application Data

WB

IHC