生物描述

特异性

CHD3 Rabbit Recombinant mAb detects endogenous levels of total CHD3.

背景

Chromatin remodeling enzymes contribute to the dynamic changes that occur in chromatin structure during cellular processes such as transcription, recombination, repair, and replication. Members of the chromodomain helicase DNA-binding (Chd) family of enzymes belong to the SNF2 superfamily of ATP-dependent chromatin remodelers. Chromatin remodeling enzymes have three subfamilies: Chd1-Chd2 subfamily, Chd3-Chd4 subfamily, and Chd5-Chd9 subfamily. Among them, the Chd3-Chd4 Subfamily, which lacks the DNA-binding domain, includes the proteins Chd3 and Chd4. CHD3 is an important component of the initial repression of the herpesvirus genome. It localizes to early viral foci and suppresses viral gene expression. Depletion of CHD3 results in enhanced viral immediate early gene expression and an increase in the number of transcriptionally active viral genomes in the cell. Importantly, CHD3 can recognize the repressive histone marks that have been detected in the chromatin associated with the viral genome and this remodeler is important for ultimately reducing the levels of accessible viral genomes. In total, CHD3 is an important epigenetic repressor of initial infection.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human Mouse Rat
MW (kDa)	260kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Concetta G.A. Marfella, et al. Mutat Res. 2007, 618(1-2): 30-40. [2] Arbuckle JH, et al. MBio. 2014, 5(1):e01027-13.

Application Data

WB

IHC

IF