生物描述

特异性

Cyclophilin A Rabbit Recombinant mAb detects endogenous levels of total Cyclophilin A.

背景

Cyclophilin A (CyPA) is a ubiquitously distributed protein belonging to the immunophilin family. CyPA has peptidyl prolyl cis-trans isomerase (PPIase) activity, which regulates protein folding and trafficking. Cyclophilins are present in all cellular compartments, involved in processes including protein trafficking and maturation, receptor complex stabilization, apoptosis, receptor signaling, RNA processing, muscle differentiation, detoxification of reactive oxygen species (ROS), immune response, spliceosome assembly, miRNA activity and RISC assembly. Human CyPs consist of 16 family members that are structurally distinct. Among them, the most abundant member is CyPA, which makes up ∼0.1–0.6% of the total cytosolic proteins. CyPA is present intracellularly and can also be secreted from cells in response to inflammatory stimuli such as hypoxia, infection, and oxidative stress. The secreted form of CyPA may mediate intercellular communication acting as an autocrine/paracrine factor. Extracellular CyPA stimulates pro-inflammatory signals in endothelial cells (EC) and vascular smooth muscle cells (VSMC), being an nonmitogenic survival-enhancing autocrine factor that contributes to mouse embryonic stem cell growth, and has a potent chemotactic effect on leukocytes, monocytes, and lymphocytes.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	18kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Sumita Kumari, et al. Plant Signal Behav. 2013, 8(1): e22734. [2] Nigro P, et al. Cell Death Dis. 2013, 4:e888. [3] Piotukh K, et al. J Biol Chem. 2005, 280(25):23668-74.

Application Data

WB

IHC