Cytokeratin 18 Rabbit Recombinant mAb Datasheet

生物描述

特异性	Cytokeratin 18 Rabbit Recombinant mAb detects endogenous level of cytokeratin 18.
背景	The intermediate filaments consist of a large number of nuclear and cytoplasmic proteins that are expressed in a tissue- and differentiation-dependent manner. The components of the intermediate filaments are highly conserved during evolution and show a high degree of conservation among species. Cytokeratins are major structural proteins found in epithelial cells, which form the cytoplasmic network of intermediate filaments. Cytokeratins consist of at least 20 unique gene products that fall into two categories: the relatively acidic type I group (CK9-CK20) and the neutral-basic type II group (CK1-CK8). Cytokeratin 18 (CK18) is a type I intermediate filament protein that is found primarily in many types of single-layered or "simple" epithelial tissues and is localized in the cytoplasm and perinuclear region. CK18 and its coexpressed complementary type II keratin partner, CK8, are persistently expressed in a variety of adult epithelial organs, such as the liver, lung, kidney, pancreas, gastrointestinal tract, and mammary gland, and are also expressed by cancers that arise from these tissues. The known function of CK18 is to provide a flexible intracellular scaffolding to structure cytoplasm, resist stresses externally applied to the cell and maintain normal mitochondrial structures. CK18 is also important for cellular processes such as apoptosis, mitosis, cell cycle progression, and cell signaling.

特异性

Cytokeratin 18 Rabbit Recombinant mAb detects endogenous level of cytokeratin 18.

背景

The intermediate filaments consist of a large number of nuclear and cytoplasmic proteins that are expressed in a tissue- and differentiation-dependent manner. The components of the intermediate filaments are highly conserved during evolution and show a high degree of conservation among species. Cytokeratins are major structural proteins found in epithelial cells, which form the cytoplasmic network of intermediate filaments. Cytokeratins consist of at least 20 unique gene products that fall into two categories: the relatively acidic type I group (CK9-CK20) and the neutral-basic type II group (CK1-CK8). Cytokeratin 18 (CK18) is a type I intermediate filament protein that is found primarily in many types of single-layered or "simple" epithelial tissues and is localized in the cytoplasm and perinuclear region. CK18 and its coexpressed complementary type II keratin partner, CK8, are persistently expressed in a variety of adult epithelial organs, such as the liver, lung, kidney, pancreas, gastrointestinal tract, and mammary gland, and are also expressed by cancers that arise from these tissues. The known function of CK18 is to provide a flexible intracellular scaffolding to structure cytoplasm, resist stresses externally applied to the cell and maintain normal mitochondrial structures. CK18 is also important for cellular processes such as apoptosis, mitosis, cell cycle progression, and cell signaling.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human

MW (kDa)

46kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Weng YR, et al. Mol Cancer Res. 2012, 10(4):485-93.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck