Daxx Rabbit Recombinant mAb Datasheet

生物描述

特异性	Daxx Rabbit Recombinant mAb detects endogenous levels of total Daxx.
背景	The death-domain-associated protein (Daxx) could be associated with both nuclear and cytoplasmic events during apoptosis. Daxx was originally identified as a protein that specifically binds to the death domain of the transmembrane death receptor FAS (also called CD95) in the cytoplasm and potentiates FAS-induced apoptosi. Daxx is ubiquitously expressed throughout the body with particular high expression in the thymus and testes. At the cellular level, Daxx is mainly a nuclear protein that associates with several different subnuclear structures, although it also interacts with FAS at the plasma membrane. Daxx interacts with pro-apoptotic receptors such as FAS and TGFb receptor II. Daxx can activate both JNK and p38, interact with and activate the upstream JNK kinase kinase ASK1 not only in response to FAS stimulation, but also upon glucose deprivation. Daxx is known to repress several transcription factors, including Pax3, ETS1, E2F1, NF-kB, p53 and p73. Moreover, Daxx interacts with and inhibits Smad4 upon TGFb stimulation. Daxx was reported to interact with several crucial proteins involved in transcriptional silencing, namely histone deacetylase II, core histones and the chromatin-associated protein DEK.

特异性

Daxx Rabbit Recombinant mAb detects endogenous levels of total Daxx.

背景

The death-domain-associated protein (Daxx) could be associated with both nuclear and cytoplasmic events during apoptosis. Daxx was originally identified as a protein that specifically binds to the death domain of the transmembrane death receptor FAS (also called CD95) in the cytoplasm and potentiates FAS-induced apoptosi. Daxx is ubiquitously expressed throughout the body with particular high expression in the thymus and testes. At the cellular level, Daxx is mainly a nuclear protein that associates with several different subnuclear structures, although it also interacts with FAS at the plasma membrane. Daxx interacts with pro-apoptotic receptors such as FAS and TGFb receptor II. Daxx can activate both JNK and p38, interact with and activate the upstream JNK kinase kinase ASK1 not only in response to FAS stimulation, but also upon glucose deprivation. Daxx is known to repress several transcription factors, including Pax3, ETS1, E2F1, NF-kB, p53 and p73. Moreover, Daxx interacts with and inhibits Smad4 upon TGFb stimulation. Daxx was reported to interact with several crucial proteins involved in transcriptional silencing, namely histone deacetylase II, core histones and the chromatin-associated protein DEK.

使用信息

抗体应用

WB, IF,ELISA,ELISA

稀释比例

WB	IF
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

47kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Salomoni P, et al. Trends Cell Biol. 2006, 16(2):97-104.

Application Data

WB

Validated by Selleck

IF

Validated by Selleck