生物描述

特异性

DNA-PKcs Rabbit Recombinant mAb detects endogenous levels of total DNA-PKcs.

背景

DNA-PKcs is a catalytic subunit of DNA-PK, a nuclear DNA-dependent serine/threonine protein kinase. DNA-PK is comprised of three key components (Ku70/80 and DNA-PKcs) and is a member of the phosphatidylinositol-3-kinase (PI3K) family. DNA-PK is involved in V(D)J recombination and also strongly implicated in telomere maintenance. Like Ku, DNA-PKcs is located at telomeres and has a role in telomere capping, however, it does not affect telomere length or telomerase activity. DNA-PKcs protects telomeric DNA and chromosome ends. DNA-PK activation and post-translational modifications are numerous and complex. The autophosphorylation of DNA-PK results in kinase inactivation and dissociation of DNA-PKcs from the heterodimer Ku. In addition, Ku (as the DNA recognition component) and DNA-PKcs could influence transcription. DNA-PK (comprising both DNA-PKcs and Ku) functions as a potent inhibitor of transcription initiation by Pol I and that this inhibition involves ATP hydrolysis and Pol I phosphorylation. In addition to RNA-Pol I, DNA-PK has also been shown to phosphorylate RNA Polymerase II (RNA-Pol II) and a variety of sequence specific regulatory transcription factors. DNA-PKcs may act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. It is autophosphorylated after cellular IR exposures, which modifies its binding with Ku and has been implicated in the phosphorylation of a wide range of DNA damage/checkpoint proteins. This auto-phosphorylation event is critical for correct NHEJ activity within the cell.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	469kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Hill R, et al. Cell Cycle. 2010, 9(17):3460-9. [2] Collis SJ, et al. Oncogene. 2005, 24(6):949-61.

Application Data

WB

IHC