Dnmt1 Rabbit Recombinant mAb

生物描述

特异性

Dnmt1 Rabbit Recombinant mAb detects endogenous level of total Dnmt1.

背景

DNA methylation is involved in epigenetic regulation of gene expression, in the context of X chromosome inactivation, genomic imprinting, and development. In the cell, Dnmt1 is the major enzyme in maintenance of the pattern of DNA methylation after DNA replication. The protein is located at the replication fork, where it could directly modify nascent DNA immediately after replication. Dnmt1 prefers hemimethylated DNA over unmethylated DNA up to 40-fold in vitro. DNMT1 is highly expressed during the S phase and mostly localized to nuclei of somatic cells during G1 and G2 phases and is actively targeted to replication foci during the S-phase. DNMT1 is found to be either downregulated or upregulated during cellular differentiation. In many instances, for example in muscle cells, F9 mouse tumor cells, PC12 neuronal cells and in skin cells DNMT1 expression and/or activity levels are decreased in cells when differentiation is induced experimentally. DNMT1 expression is upregulated in certain malignant blood cells. DNMT1 is also shown to contribute to the cellular response to DNA damage. DNMT1 is recruited rapidly and transiently to DNA double strand breaks and interacts with multiple components of the DNA damage response machinery including PCNA and the ATR effector kinase CHK1. DNMT is highly expressed in cancer cells and associated with cellular transformation.

使用信息

抗体应用	WB, IF,ELISA,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	183kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Hermann A, et al. J Biol Chem. 2004, 279(46):48350-9. [2] Sirano Dhe-Paganon, et al. Int J Biochem Mol Biol. 2011, 2(1): 58–66.

Application Data

WB

IF