生物描述

特异性

E2F4 Rabbit Recombinant mAb detects endogenous levels of total E2F4.

背景

The E2F family of transcription factors is a key determinant of cell proliferation in response to extra- and intra-cellular signals. Within this family, E2F4 is a transcriptional repressor whose activity is critical to engage and maintain cell cycle arrest in G0/G1 in conjunction with members of the retinoblastoma (RB) family. E2F4 has a multitude of functions in cells besides this cell cycle regulatory role, including in embryonic and adult stem cells, during regenerative processes, and in cancer. The activity of E2F4 is regulated by several mechanisms such as sub-cellular localization, interactions with retinoblastoma (RB) proteins, post-translational modification such as phosphorylation, and decreased translation mediated by antisense transcripts. Unlike E2F1, E2F4 does not have a nuclear localization signal (NLS) and primarily exists in the cytoplasm during cell cycle progression. Upon cell cycle arrest, due to the depletion of mitogenic stimuli, cells start to enter into G0, and cytoplasmic E2F4 forms heterodimers with a DP protein, which facilitates the localization of E2F4 complexes into the nucleus in a CRM1 mediated manner. Nuclear localized E2F4 complexes occupy target promoters and regulate diverse classes of genes which are involved in cell cycle, DNA repair and apoptosis.

使用信息

抗体应用	WB, IF,ELISA,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human
MW (kDa)	60kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Jenny Hsu, et al. Cell Cycle. 2016, 15(23): 3183–3190. [2] Lee BK, et al. Nucleic Acids Res. 2011, 39(9):3558-73.

Application Data

WB

IF