Datasheet

生物描述

特异性	EEA1 Rabbit Recombinant mAb detects endogenous levels of total EEA1.
背景	EEA1 is an early endosomal Rab5 effector protein that has been implicated in the docking of incoming endocytic vesicles before fusion with early endosomes. Unlike many other endosomal proteins, EEA1 has not been detected on other membrane compartments and appears to be one of the most specific early endosomal markers known to date. EEA1 labels a subdomain of the early endosome and does not associate with the recycling endosome. EEA1 is the only Rab5 effector that could confer minimal fusion activity in a reconstituted endosomal fusion assay and plays a docking/tethering role before SNARE-dependent fusion.

特异性

EEA1 Rabbit Recombinant mAb detects endogenous levels of total EEA1.

背景

EEA1 is an early endosomal Rab5 effector protein that has been implicated in the docking of incoming endocytic vesicles before fusion with early endosomes. Unlike many other endosomal proteins, EEA1 has not been detected on other membrane compartments and appears to be one of the most specific early endosomal markers known to date. EEA1 labels a subdomain of the early endosome and does not associate with the recycling endosome. EEA1 is the only Rab5 effector that could confer minimal fusion activity in a reconstituted endosomal fusion assay and plays a docking/tethering role before SNARE-dependent fusion.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

170kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Jean M. Wilson, et al. Mol Biol Cell. 2000, 11(8): 2657–2671.

Application Data

WB

Validated by Selleck