生物描述

特异性

eEF1A1 Rabbit Recombinant mAb detects endogenous levels of total eEF1A1

背景

The eukaryotic translation elongation factors 1 alpha, eEF1A1 and eEF1A2, are the second most abundant protein (1–3% of total protein content) after actin and an important component of translation machinery. The cellular expression of eEF1A is divided into three classes. First are the majority of cell types that express only eEF1A1. Second, neurons and muscle cells that express only eEF1A2. Third class belongs to certain tumor cell types and cell lines that express both eEF1A isoforms. eEF1A1 protein shares homology with eEF1A2 protein at the nucleotide level (75%) and amino acid level (96%). eEF1A2 does not bind GDP and GTP with the same relative affinity as eEF1A1. The GDP dissociation rate constant is seven times higher for eEF1A1 than for eEF1A2. In addition, the nucleotide preference ratio (GDP/GTP) for eEF1A1 is 0.82 and for eEF1A2 is 1.50. Furthermore, eEF1A has been shown to be a novel component of the nuclear export machinery in mammalian cells and is involved in the nuclear export of specific proteins such as VHL van Hippel-Lindau (VHL) tumor suppressor and poly(A)-binding protein (PABP1).

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	50kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Abbas W, et al. Front Oncoll. 2015,7;5:75.

Application Data

WB

IHC