EEF2 Rabbit Recombinant mAb

生物描述

特异性

EEF2 Rabbit Recombinant mAb detects endogenous level of total EEF2.

背景

Eukaryotic elongation factor 2 (EEF2) is a monomeric protein, with a mass of 95.2 kDa, having 857 amino acid residues and is located in cytoplasm. It is a member of the G-protein super family which undergoes conformational changes associated with binding of the guanosine nucleotide and hydrolysis of the bound GTP. eEF2 regulates peptide-chain elongation. The eEF2 activity determines the translocation step of protein synthesis thus regulating the protein synthesis. Phosphorylation of eEF2 inhibits its activity by preventing it from binding to the ribosome thus affecting the translocation and poly (U)-directed polyphenylalanine synthesis. The regulation at the level of translation elongation in eukaryotes seems to be one of the important steps in regulating protein synthesis. This step is mainly regulated through activation and deactivation of eEF2.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human Mouse Rat
MW (kDa)	95kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Kaul G, et al. Cell Biochem Funct. 2011, 29(3):227-34.

Application Data

WB

IHC

IF