EIF4E Rabbit Recombinant mAb

生物描述

特异性

EIF4E Rabbit Recombinant mAb detects endogenous level of total EIF4E.

背景

Control of mRNA translation plays a critical role in cell growth, proliferation and differentiation. In eukaryotes, most mRNAs are translated in a cap-dependent manner. The cap structure m7GpppN (where N is any nucleotide) is found at the 5' terminus of all cellular eukaryotic mRNAs (except those in organelles). The mRNA 5' cap-binding protein eIF4E is a key player in the regulation of translation. eIF4E is regulated at multiple levels, including gene expression, sequestration and phosphorylation, etc. It mediates a rate-limiting process that drives selective translation of many oncongenic proteins such as cyclin D1, survivin and VEGF, thereby contributing to tumour growth, metastasis and therapy resistance.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human Mouse Rat
MW (kDa)	30kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Mamane Y, et al. Oncogene. 2004, 23(18):3172-9. [2] Lu C, et al. Expert Rev Mol Med. 2016, 18:e2.

Application Data

WB

IHC

IF