14-3-3 sigma Rabbit Recombinant mAb

生物描述

特异性

ERK5 Rabbit Recombinant mAb detects endogenous level of total ERK5.

背景

Extracellular-signal-regulated kinase 5 (ERK5) is a member of the mitogen-activated protein kinase (MAPK) family and, similar to ERK1/2, has the Thr–Glu–Tyr (TEY) activation motif. Both ERK5 and ERK1/2 are activated by growth factors and have an important role in the regulation of cell proliferation and cell differentiation. ERK5 is expressed in many tissue types, but is particularly abundant in the heart, skeletal muscle, placenta, lungs and kidneys. ERK5 is ubiquitously expressed in various different cell lines and is localised in both nuclear and cytoplasmic compartments. On stimulation, MEKK2 and MEKK3, members of the MAPKKK family, activate MEK5, a specific MAPKK for ERK5, although these two MAPKKKs are associated differently with upstream signalling pathways. Subsequently, MEK5 phosphorylates and activates ERK5, and then the activated ERK5 phosphorylates substrates including MEF2. The interaction of MEK5 with MEKK2, MEKK3 or ERK5 is mediated by the PB1 domain of MEK5. The ERK5 signalling axis has been implicated in normal physiological development of the cardiovascular system and in pathological conditions such as ischaemia, chronic pain and cancer.

使用信息

抗体应用	WB, IF,ELISA,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	115kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Satoko Nishimoto, et al. EMBO Rep. 2006, 7(8): 782–786. [2] Nithianandarajah-Jones GN, et al. Cell Signal. 2012, 24(11):2187-96.

Application Data

WB

IF