FOXO3A Rabbit Recombinant mAb Datasheet

生物描述

特异性	FOXO3A Rabbit Recombinant mAb detects endogenous levels of total FOXO3A.
背景	Forkhead box-O (FoxO) family transcriptional factors control the expression of many genes involved in a variety of cellular processes such as the cell cycle, apoptosis, DNA repair, energy metabolism, development, and protection from oxidative stress. Four members of the mammalian FoxO family have been identified: FoxO1, FoxO3a, FoxO4, and FoxO6. FoxO3a regulates many target genes that are involved in the antioxidative response in various cell types, including skin cells. It is a key factor in oxidative stress-related cellular responses in melanogenesis. Foxo3a has a highly conserved forkhead domain with a winged-helix motif and measurable DNA-binding activity. This domain functions as a transcription factor for various genes, including p27/Kip1, p130-Rb2, and cyclin D1/2 (cell-cycle regulation), tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), Bim, and Fas ligand (apoptosis), and GADD45a (DNA repair). The function and localization of FOXO3A depends on its phosphorylation status. FOXO3A is phosphorylated when cells are stimulated with serum or growth factors. FOXO3A is then exported from the nucleus to the cytoplasm, where it interacts with 14-3-3 proteins, resulting in its loss of function as a transcription factor. By contrast, the dephosphorylated form of FOXO3A translocates into the nucleus and activates target genes when cells are deprived of serum or growth factors. Thus, the transcriptional activity of FOXO3A is negatively regulated by phosphorylation. FOXO3A is phosphorylated primarily by a serine/threonine kinase, AKT, at threonine 32 (T32), serine 253 (S253), and serine 315 (S315) residues.

特异性

FOXO3A Rabbit Recombinant mAb detects endogenous levels of total FOXO3A.

背景

Forkhead box-O (FoxO) family transcriptional factors control the expression of many genes involved in a variety of cellular processes such as the cell cycle, apoptosis, DNA repair, energy metabolism, development, and protection from oxidative stress. Four members of the mammalian FoxO family have been identified: FoxO1, FoxO3a, FoxO4, and FoxO6. FoxO3a regulates many target genes that are involved in the antioxidative response in various cell types, including skin cells. It is a key factor in oxidative stress-related cellular responses in melanogenesis. Foxo3a has a highly conserved forkhead domain with a winged-helix motif and measurable DNA-binding activity. This domain functions as a transcription factor for various genes, including p27/Kip1, p130-Rb2, and cyclin D1/2 (cell-cycle regulation), tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), Bim, and Fas ligand (apoptosis), and GADD45a (DNA repair). The function and localization of FOXO3A depends on its phosphorylation status. FOXO3A is phosphorylated when cells are stimulated with serum or growth factors. FOXO3A is then exported from the nucleus to the cytoplasm, where it interacts with 14-3-3 proteins, resulting in its loss of function as a transcription factor. By contrast, the dephosphorylated form of FOXO3A translocates into the nucleus and activates target genes when cells are deprived of serum or growth factors. Thus, the transcriptional activity of FOXO3A is negatively regulated by phosphorylation. FOXO3A is phosphorylated primarily by a serine/threonine kinase, AKT, at threonine 32 (T32), serine 253 (S253), and serine 315 (S315) residues.

使用信息

抗体应用

WB, IF, IHC,ELISA,ELISA

稀释比例

WB	IHC	IF
1:1000	1:50	1:50

反应性

Human

MW (kDa)

90kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck

IF

Validated by Selleck