Galectin 3 Rabbit Recombinant mAb Datasheet

生物描述

特异性	Galectin 3 Rabbit Recombinant mAb detects endogenous level of Galectin 3.
背景	Galectins are a group of evolutionarily conserved proteins with the ability to bind β-galactosides through characteristic carbohydrate-recognition domains (CRD). Galectin-3 (Gal-3) is a pleiotropic lectin that plays an important role in cell proliferation, adhesion, differentiation, angiogenesis, and apoptosis. Galectin-3 expression in tissues appears to be developmentally regulated, being more abundant during embryogenesis and development than in the adult life, when it is detected in various epithelial cells, cartilage and bone as well as in inflammatory cells, either constitutively or in an inducible fashion. Galectin-3 shows a ubiquitous localization within the cell. galectin-3 is also secreted into the extracellular space, where it is found both on the cell surface and in the extracellular space. Its intracellular distribution depends on the cell cycle, being mainly cytoplasmic in quiescent cells, and nuclear in replicating cells. Extracellular galectin-3 interacts with the β-galactoside residues of several extracellular matrix (ECM) and cell surface glycoproteins via the CRD. This is the classical lectin-glycoconjugate interaction. It also regulates cell adhesion. Intracellular galectin-3 acts as a pre-mRNA splicing factor and also regulates the cell cycle, through a G1 or G2/M arrest, via downregulation of cyclin A and E, upregulation of p21 and p27 cyclin inhibitors and hypophosphorylation of Rb protein. Moreover, galectin-3 has been shown to be a key regulator of the Wnt/β-catenin signaling pathway because of its ability to interact with β-catenin and translocate it into the nucleus, with consequent stimulation of cyclin D1 and c-myc expression.

特异性

Galectin 3 Rabbit Recombinant mAb detects endogenous level of Galectin 3.

背景

Galectins are a group of evolutionarily conserved proteins with the ability to bind β-galactosides through characteristic carbohydrate-recognition domains (CRD). Galectin-3 (Gal-3) is a pleiotropic lectin that plays an important role in cell proliferation, adhesion, differentiation, angiogenesis, and apoptosis. Galectin-3 expression in tissues appears to be developmentally regulated, being more abundant during embryogenesis and development than in the adult life, when it is detected in various epithelial cells, cartilage and bone as well as in inflammatory cells, either constitutively or in an inducible fashion. Galectin-3 shows a ubiquitous localization within the cell. galectin-3 is also secreted into the extracellular space, where it is found both on the cell surface and in the extracellular space. Its intracellular distribution depends on the cell cycle, being mainly cytoplasmic in quiescent cells, and nuclear in replicating cells. Extracellular galectin-3 interacts with the β-galactoside residues of several extracellular matrix (ECM) and cell surface glycoproteins via the CRD. This is the classical lectin-glycoconjugate interaction. It also regulates cell adhesion. Intracellular galectin-3 acts as a pre-mRNA splicing factor and also regulates the cell cycle, through a G1 or G2/M arrest, via downregulation of cyclin A and E, upregulation of p21 and p27 cyclin inhibitors and hypophosphorylation of Rb protein. Moreover, galectin-3 has been shown to be a key regulator of the Wnt/β-catenin signaling pathway because of its ability to interact with β-catenin and translocate it into the nucleus, with consequent stimulation of cyclin D1 and c-myc expression.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human Rat

MW (kDa)

30kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck