GRK2 Rabbit Recombinant mAb Datasheet

生物描述

特异性	GRK2 Rabbit Recombinant mAb detects endogenous level of total GRK2.
背景	GRK2 is a ubiquitous member of the G protein-coupled receptor kinase (GRK) family that appears to play a central, integrative role in signal transduction cascades. GRKs participate together with arrestins in the regulation of G protein-coupled receptors (GPCR), a family of hundreds of membrane proteins of key physiological and pharmacological importance, by triggering receptor desensitization from G proteins and GPCR internalization, and also by helping assemble macromolecular signalosomes in the receptor environment acting as agonist-regulated adaptor scaffolds, thus contributing to signal propagation. GRK family members can be subdivided into three main groups based on sequence homology: visual GRK subfamily (GRK1 and GRK7), the β-adrenergic receptor kinase subfamily (GRK2/GRK3) and the GRK4 subfamily (GRK4, GRK5 and GRK6). GRK2, 3, 5 and 6 are ubiquitously expressed in mammalian tissues. GRK2 can phosphoryate non-GPCR substrates and display a complex network of functional interactions. GRK2 contributes to orchestrate G2/M checkpoint mechanisms, helping to restrict the apoptotic fate of arrested cells.

特异性

GRK2 Rabbit Recombinant mAb detects endogenous level of total GRK2.

背景

GRK2 is a ubiquitous member of the G protein-coupled receptor kinase (GRK) family that appears to play a central, integrative role in signal transduction cascades. GRKs participate together with arrestins in the regulation of G protein-coupled receptors (GPCR), a family of hundreds of membrane proteins of key physiological and pharmacological importance, by triggering receptor desensitization from G proteins and GPCR internalization, and also by helping assemble macromolecular signalosomes in the receptor environment acting as agonist-regulated adaptor scaffolds, thus contributing to signal propagation. GRK family members can be subdivided into three main groups based on sequence homology: visual GRK subfamily (GRK1 and GRK7), the β-adrenergic receptor kinase subfamily (GRK2/GRK3) and the GRK4 subfamily (GRK4, GRK5 and GRK6). GRK2, 3, 5 and 6 are ubiquitously expressed in mammalian tissues. GRK2 can phosphoryate non-GPCR substrates and display a complex network of functional interactions. GRK2 contributes to orchestrate G2/M checkpoint mechanisms, helping to restrict the apoptotic fate of arrested cells.

使用信息

抗体应用

WB, IHC, IF,ELISA,ELISA

稀释比例

WB	IHC	IF
1:1000	1:50	1:50

反应性

Human Mouse Rat

MW (kDa)

80kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck

IF

Validated by Selleck