HDAC3 Rabbit Recombinant mAb Datasheet

生物描述

特异性	HDAC3 Rabbit Recombinant mAb detects endogenous level of total HDAC3.
背景	Histone deacetylase 3 (HDAC3) is one of four members of the human class I HDACs (class I HDACs include HDAC1, HDAC2, HDAC3, and HDAC8) that regulates gene expression by deacetylation of histones and nonhistone proteins. HDAC3 is located in both the nucleus and in the cytoplasm and can self-associate to form dimers and trimers in vitro and in vivo. HDAC3 represses transcription when directed to promoter regions by serving as a corepressor. HDAC1 binds HDAC2 to form the catalytic core of the Sin3, Mi2/NuRD and CoREST complexes, whereas HDAC3 is the catalytic subunit of the N-CoR and SMRT complexes. HDAC3 is the most highly expressed class I HDAC throughout the brain, including the hippocampus. It alters gene expression as part of a large complex that contains corepressors, nuclear receptor corepressor 1 (NCoR) and silencing mediator for retinoid and thyroid-hormone receptors (SMRT), as well as class IIa HDACs, such as HDAC4.

特异性

HDAC3 Rabbit Recombinant mAb detects endogenous level of total HDAC3.

背景

Histone deacetylase 3 (HDAC3) is one of four members of the human class I HDACs (class I HDACs include HDAC1, HDAC2, HDAC3, and HDAC8) that regulates gene expression by deacetylation of histones and nonhistone proteins. HDAC3 is located in both the nucleus and in the cytoplasm and can self-associate to form dimers and trimers in vitro and in vivo. HDAC3 represses transcription when directed to promoter regions by serving as a corepressor. HDAC1 binds HDAC2 to form the catalytic core of the Sin3, Mi2/NuRD and CoREST complexes, whereas HDAC3 is the catalytic subunit of the N-CoR and SMRT complexes. HDAC3 is the most highly expressed class I HDAC throughout the brain, including the hippocampus. It alters gene expression as part of a large complex that contains corepressors, nuclear receptor corepressor 1 (NCoR) and silencing mediator for retinoid and thyroid-hormone receptors (SMRT), as well as class IIa HDACs, such as HDAC4.

使用信息

抗体应用

WB ,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

49kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck