IRF1 Rabbit Recombinant mAb Datasheet

生物描述

特异性	IRF1 Rabbit Recombinant mAb detects endogenous level of total IRF1.
背景	Interferon Regulatory Factor 1 (IRF1) is an essential player in many facets of the immune response and oncogenesis. The IRF1 protein is also very unstable (half life ~30 min) compared with IRF2 (half-life ~8 hrs). IRF1 is expressed at low levels in unstimulated cells and is activated by many cytokines including type I (IFN , IFN , and others) and II (IFN ) interferons, tumor necrosis factor- (TNF- ), retinoic acid, interleukin-1 (IL-1), IL-6, and viral infection. Initial signaling is mediated through the Janus-activated kinase-signal transducer and activator of transcription (JAK/STAT1) pathway, leading to the activation of the IRF1 promoter by the Stat and NF-κB transcription factors. When a signal is transduced through the IFN receptor, phosphorylated STAT1 translocates to the nucleus where it induces the transcription of primary IFNγ response genes [20]. Stat1-deficient cells no longer respond to IFN stimulation by inducing IRF1 expression. IRF1 has remarkable functional diversity and controls the transcription of genes involved in mediating antiviral, immunomodulatory, and antiproliferative effects, also involved in cell cycle regulation and apoptosis in response to a variety of stressors.

特异性

IRF1 Rabbit Recombinant mAb detects endogenous level of total IRF1.

背景

Interferon Regulatory Factor 1 (IRF1) is an essential player in many facets of the immune response and oncogenesis. The IRF1 protein is also very unstable (half life ~30 min) compared with IRF2 (half-life ~8 hrs). IRF1 is expressed at low levels in unstimulated cells and is activated by many cytokines including type I (IFN , IFN , and others) and II (IFN ) interferons, tumor necrosis factor- (TNF- ), retinoic acid, interleukin-1 (IL-1), IL-6, and viral infection. Initial signaling is mediated through the Janus-activated kinase-signal transducer and activator of transcription (JAK/STAT1) pathway, leading to the activation of the IRF1 promoter by the Stat and NF-κB transcription factors. When a signal is transduced through the IFN receptor, phosphorylated STAT1 translocates to the nucleus where it induces the transcription of primary IFNγ response genes [20]. Stat1-deficient cells no longer respond to IFN stimulation by inducing IRF1 expression. IRF1 has remarkable functional diversity and controls the transcription of genes involved in mediating antiviral, immunomodulatory, and antiproliferative effects, also involved in cell cycle regulation and apoptosis in response to a variety of stressors.

使用信息

抗体应用

WB, IF,ELISA,ELISA

稀释比例

WB	IF
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

48kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] J. L. Schwartz, et al. International Journal of Breast Cancer. 2011.

Application Data

WB

Validated by Selleck

IF

Validated by Selleck