生物描述

特异性

M6PR Rabbit Recombinant mAb detects endogenous levels of total M6PR.

背景

The mannose 6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2R) encodes a multifunctional protein involved in lysosomal enzyme trafficking, fetal organogenesis, tumor suppression, and T cell- mediated immunity. It interacts with a diverse group of ligands not only intracellularly, but also at the cell surface. These ligands include numerous lysosomal enzymes, the latent complex of transforming growth factor-β (TGF-β), and granzyme B. In addition to binding M6P-modified glycoproteins, M6P/IGF2R also interacts specifically with several molecules through M6P-independent mechanisms. The best characterized of these is the mitogen insulin-like growth factor 2 (IGF2). It binds to a M6P-independent, high-affinity receptor binding site. Bound IGF2 is then internalized and transported to the lysosomes where it is degraded; M6P/IGF2R is subsequently recycled back to the membrane. Thus, M6P/IGF2R controls the extracellular bioavailability of IGF2 and TGFβ, thereby regulating cell proliferation and apoptosis.

使用信息

抗体应用	WB, IF,ELISA,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	274kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Andrew A. Wylie, et al. Am J Pathol. 2003, 162(1): 321–328.

Application Data

WB

IF