MMP8 Rabbit Recombinant mAb Datasheet

生物描述

特异性	MMP8 Rabbit Recombinant mAb detects endogenous level of total MMP8.
背景	Matrix metalloproteinases (MMPs) comprise a large family of zinc-dependent endoproteinases, collectively capable of degrading all extracellular matrix (ECM) components. They are synthesized as zymogens with a signal peptide which leads them to the secretory pathway, and then, they are secreted from the cell or anchored to the plasma membrane thereby confining their catalytic activity to the extracellular space or to the cell surface. Based on their domain organization, MMPs can be classified in four different groups: archetypal MMPs, matrilysins, gelatinases and furin-activatable MMPs. The archetypal MMPs then have been subdived into three different subgroups: collagenases, stromelysins and other archetypal MMPs. MMP8 belongs to teh group of collagenases which include MMP-1 and MMP-13 besides MMP8. Collagenases are also able to proteolytically process other ECM proteins, as well as a number of bioactive molecules such as interleukin-8 (IL-8), pro-tumor necrosis factor (TNF)-α, protease-activated receptor-1, and several insulin-like growth factor binding proteins (IGFBPs). The cooperation between the catalytic and hemopexin domains is essential to carry out their collagenolytic activity. They each has preferential activity against one of the three major types of fibrillar collagen: MMP-1 is mainly active against type III collagen, MMP-8 targets type I collagen and MMP-13 preferentially cleaves type II collagen

特异性

MMP8 Rabbit Recombinant mAb detects endogenous level of total MMP8.

背景

Matrix metalloproteinases (MMPs) comprise a large family of zinc-dependent endoproteinases, collectively capable of degrading all extracellular matrix (ECM) components. They are synthesized as zymogens with a signal peptide which leads them to the secretory pathway, and then, they are secreted from the cell or anchored to the plasma membrane thereby confining their catalytic activity to the extracellular space or to the cell surface. Based on their domain organization, MMPs can be classified in four different groups: archetypal MMPs, matrilysins, gelatinases and furin-activatable MMPs. The archetypal MMPs then have been subdived into three different subgroups: collagenases, stromelysins and other archetypal MMPs. MMP8 belongs to teh group of collagenases which include MMP-1 and MMP-13 besides MMP8. Collagenases are also able to proteolytically process other ECM proteins, as well as a number of bioactive molecules such as interleukin-8 (IL-8), pro-tumor necrosis factor (TNF)-α, protease-activated receptor-1, and several insulin-like growth factor binding proteins (IGFBPs). The cooperation between the catalytic and hemopexin domains is essential to carry out their collagenolytic activity. They each has preferential activity against one of the three major types of fibrillar collagen: MMP-1 is mainly active against type III collagen, MMP-8 targets type I collagen and MMP-13 preferentially cleaves type II collagen

使用信息

抗体应用

WB ,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

53kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Fanjul-Fernández M, et al. Biochim Biophys Acta. 2010, 1803(1):3-19

Application Data

WB

Validated by Selleck