Datasheet

生物描述

特异性	Myoglobin Rabbit Recombinant mAb detects endogenous levels of total Myoglobin.
背景	Myoglobin is a cytoplasmic hemoprotein, expressed solely in cardiac myocytes and oxidative skeletal muscle fibers, that reversibly binds O2 by its heme residue, a porphyrin ring:iron ion complex. It is well accepted as an O2-storage protein in muscle, capable of releasing O2 during periods of hypoxia or anoxia. Myoglobin is also thought to buffer intracellular O2 concentration when muscle activity increases and to facilitate intracellular O2 diffusion by providing a parallel path that augments simple diffusion of dissolved O2. In newborn mice, myoglobin is expressed at low levels in hindlimb muscles; however, as development proceeds and locomotor activity increases, myoglobin expression increases dramatically in oxidative, fatigue-resistant fibers. Neonatal dolphins, penguins and seals have myoglobin levels in locomotor muscles that are a fraction of those seen in adults. Myoglobin is able to bind NO and may serve as an important scavenger of NO in heart and oxidative skeletal muscle. Myoglobin is also known to have peroxidase activity.

特异性

Myoglobin Rabbit Recombinant mAb detects endogenous levels of total Myoglobin.

背景

Myoglobin is a cytoplasmic hemoprotein, expressed solely in cardiac myocytes and oxidative skeletal muscle fibers, that reversibly binds O2 by its heme residue, a porphyrin ring:iron ion complex. It is well accepted as an O2-storage protein in muscle, capable of releasing O2 during periods of hypoxia or anoxia. Myoglobin is also thought to buffer intracellular O2 concentration when muscle activity increases and to facilitate intracellular O2 diffusion by providing a parallel path that augments simple diffusion of dissolved O2. In newborn mice, myoglobin is expressed at low levels in hindlimb muscles; however, as development proceeds and locomotor activity increases, myoglobin expression increases dramatically in oxidative, fatigue-resistant fibers. Neonatal dolphins, penguins and seals have myoglobin levels in locomotor muscles that are a fraction of those seen in adults. Myoglobin is able to bind NO and may serve as an important scavenger of NO in heart and oxidative skeletal muscle. Myoglobin is also known to have peroxidase activity.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:500

反应性

Human Mouse Rat

MW (kDa)

17kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Ordway GA, et al. J Exp Biol. 2004, 207(Pt 20):3441-6.

Application Data

WB

Validated by Selleck