NRF1 Rabbit Recombinant mAb

生物描述

特异性

NRF1 Rabbit Recombinant mAb detects endogenous level of total NRF1.

背景

The nuclear respiratory factors, NRF-1 and NRF-2, contribute to the expression of respiratory subunits and mitochondrial transcription factors and thus have been implicated in nucleo-mitochondrial interactions. NRF-1 could activate cytochrome c. NRF-1 has a unique DNA-binding domain, binds its recognition site as a homodimer, and functions as a positive regulator of transcription. It has a carboxy-terminal transcriptional activation domain composed of glutamine-containing clusters of hydrophobic amino acid residues that are essential for maximal activity. In proliferating mammalian cells, NRF-1 is serine phosphorylated within a concise amino-terminal domain, and phosphorylation within this domain has been associated with enhancement of both its DNA-binding activity and trans-activation functions. NRF-1 is a key regulator of nucleus-encoded subunits of all five respiratory complexes. In addition, it has a potential integrative function in coordinating bi-genomic respiratory subunit expression through its control over major regulators of mitochondrial transcription, including Tfam and both mtTFB isoform genes.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human Mouse Rat
MW (kDa)	68kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Blesa JR, et al. Biochemistry and Cell Biology. 2008, 86(1): 46-56. [2] Richard C. Scarpulla. Ann N Y Acad Sci. 2008, 1147: 321-334.

Application Data

WB

IHC

IF