p73 Rabbit Recombinant mAb

生物描述

特异性

p73 Rabbit Recombinant mAb detects endogenous level of total p73.

背景

p73 is a transcription factor and a member of the p53-family, which includes p53 itself and p63. p73 shows many p53-like properties: it could bind to p53 DNA target sites, transactivate p53-responsive genes and induce cell cycle arrest or apoptosis. There exist target genes that are specifically regulated by p73 but not by p53. c-Abl is responsible for p73 phosphorylation and stabilization upon DNA damagep. DNA damage induces c-Abl phosphorylation of Tyrosine 99 of p73. However, c-Abl also activates p38 kinase, and inhibition of p38 impairs p73 stabilization, even upon tyr99 phosphorylation. p73 phosphorylation by cyclin/CDK complexes inhibits p73 activity. The cycD/CDK4 complex, which is involved in G1 progression, is unable to phosphorylate p73, whereas CycA/CDK2, CycE/CDK2, and CycB/cdc2 (involved in S-phase and G2/M progression) phosphorylate p73 both in vivo and in vitro.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	70kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Alessandro Rufini, et al. Genes Cancer. 2011, 2(4): 491-502. [2] Stiewe T, et al. Cell Death Differ. 2002, 9(3):237-45.

Application Data

WB

IHC