生物描述

特异性

PELP1 Rabbit Recombinant mAb detects endogenous levels of total PELP1.

背景

Proline-, glutamic acid-, and leucine-rich protein 1 (PELP1) is a scaffolding protein that functions as a coregulator of several transcription factors and nuclear receptors. It is a member of chromatin remodeling complexes and plays essential roles in several pathways including hormonal signaling, cell cycle progression, ribosomal biogenesis, and the DNA damage response. PELP1 is differentially expressed in human tissues with the highest expression observed in testis, mammary gland, brain, skeletal muscle and lung tissue. In the mouse, PELP1 is expressed in the hypothalamus, testis, ovary, uterus, adrenal gland, lung, pituitary, mammary gland, cerebellum and spleen. Its cytoplasmic and nuclear subcellular localization varied depending on the tissue type. During murine mammary gland development, PELP1 expression is elevated during pregnancy and then reduced during lactation. PELP1 is a driver of proliferation in hormonal tissues and cancer. It has also been implicated in autophagy of cancer cell lines. PELP1 signaling pathways modulate cellular proliferation, migration and invasion, and hormone resistance.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human
MW (kDa)	160kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Brian J. Girard, et al. Mol Cell Endocrinol. 2014, 382(1): 10.1016/j.mce.2013.07.031. [2] Sareddy GR, et al. Gene. 2016, 585(1):128-134.

Application Data

WB

IHC