Datasheet

生物描述

特异性	PHF8 Rabbit Recombinant mAb detects endogenous levels of total PHF8.
背景	PHF8 is a histone demethylase with specificity for repressive modifications. It is an X-linked histone demethylase containing a plant homeodomain and a catalytic Jumonji C (JMJC) domain. PHF8 acts as a transcriptional activator through its ability to demethylate the repressive histone modifications H3K9me1/2, H3K27me2 and H4K20me1. In addition to its role as a histone demethylase, PHF8 functions as a chromatin reader by directly binding H3K4me3 through a pocket in its plant homeodomain finger, enabling recruitment to chromatin and allosteric activation of demethylase activity. PHF8 could regulate cell cycle progression and is necessary for the expression of many genes, including cell-cycle genes, Notch1-responsive genes and cell structure genes. PHF8 is a ubiquitously expressed nuclear protein whose dysfunction is implicated in disease. It is highly and widely expressed in many tissues, such as testis, lung and tumor tissues. Defects in PHF8 are associated with diseases such as cleft lip, cleft palate, X-linked mental retardation (XLMR) and pathogenesis of autism.

特异性

PHF8 Rabbit Recombinant mAb detects endogenous levels of total PHF8.

背景

PHF8 is a histone demethylase with specificity for repressive modifications. It is an X-linked histone demethylase containing a plant homeodomain and a catalytic Jumonji C (JMJC) domain. PHF8 acts as a transcriptional activator through its ability to demethylate the repressive histone modifications H3K9me1/2, H3K27me2 and H4K20me1. In addition to its role as a histone demethylase, PHF8 functions as a chromatin reader by directly binding H3K4me3 through a pocket in its plant homeodomain finger, enabling recruitment to chromatin and allosteric activation of demethylase activity. PHF8 could regulate cell cycle progression and is necessary for the expression of many genes, including cell-cycle genes, Notch1-responsive genes and cell structure genes. PHF8 is a ubiquitously expressed nuclear protein whose dysfunction is implicated in disease. It is highly and widely expressed in many tissues, such as testis, lung and tumor tissues. Defects in PHF8 are associated with diseases such as cleft lip, cleft palate, X-linked mental retardation (XLMR) and pathogenesis of autism.

使用信息

抗体应用

WB, IF,ELISA,ELISA

稀释比例

WB	IF
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

140kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[4] Yu L, et al. Cell Res. 2010, 20(2):166-73.

+ Expand

Application Data

WB

Validated by Selleck

IF

Validated by Selleck