Datasheet

生物描述

特异性	Phospho-ATF2 (T71) Rabbit Recombinant mAb detects endogenous levels of phosphorylated ATF2 (T71).
背景	Activating transcription factor 2 (ATF2), also known as cyclic AMP (cAMP) response element (CRE) binding protein 2 (CREB2) and CRE-BP1, is a member of the activating protein-1 (AP1) transcription factor family that regulates the expression of many genes through homo-dimerization or hetero-dimerization with other AP1 family members, such as the CREB, Fos, Maf, or Jun family transcription factors. ATF2 plays an important role in transducing extracellular signals to the nucleus to facilitate transcriptional responses to stimuli. ATF2 can be activated by many stimuli, including growth factors, ultraviolet (UV) radiation, and cytokines. ATF2 transcriptional activation is mediated by stress-activated protein kinases (SAPKs) through phosphorylation at amino acids threonine-69 and threonine-71 (T69, T71). Following phosphorylation at T69/T71, ATF2 can interact with other AP1 proteins and translocate to the nucleus to modulate the expression of hundreds of genes. Phosphorylation at Thr69 and Thr71 of ATF2 appears to enhance the intrinsic histone acetyltransferase activity of ATF2 and to regulate its degradation by the ubiquitin pathway

特异性

Phospho-ATF2 (T71) Rabbit Recombinant mAb detects endogenous levels of phosphorylated ATF2 (T71).

背景

Activating transcription factor 2 (ATF2), also known as cyclic AMP (cAMP) response element (CRE) binding protein 2 (CREB2) and CRE-BP1, is a member of the activating protein-1 (AP1) transcription factor family that regulates the expression of many genes through homo-dimerization or hetero-dimerization with other AP1 family members, such as the CREB, Fos, Maf, or Jun family transcription factors. ATF2 plays an important role in transducing extracellular signals to the nucleus to facilitate transcriptional responses to stimuli. ATF2 can be activated by many stimuli, including growth factors, ultraviolet (UV) radiation, and cytokines. ATF2 transcriptional activation is mediated by stress-activated protein kinases (SAPKs) through phosphorylation at amino acids threonine-69 and threonine-71 (T69, T71). Following phosphorylation at T69/T71, ATF2 can interact with other AP1 proteins and translocate to the nucleus to modulate the expression of hundreds of genes. Phosphorylation at Thr69 and Thr71 of ATF2 appears to enhance the intrinsic histone acetyltransferase activity of ATF2 and to regulate its degradation by the ubiquitin pathway

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human

MW (kDa)

70kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck