生物描述

特异性

Phospho-c-Myc (S62) Rabbit Recombinant mAb detects endogenous levels of total Phospho-c-Myc (S62).

背景

The c-Myc oncoprotein is a pleiotropic transcription factor involved in controlling many cellular functions, including cell proliferation, cell growth, and cell differentiation, as well as pathways that regulate genome stability and cell death. c-Myc is constitutively highly expressed in most human tumors and high expression of c-Myc in animal models can drive tumorigenesis. Expression of the c-Myc oncoprotein is affected by conserved Threonine 58 (T58) and Serine 62 (S62) phosphorylation sites that help to regulate c-Myc protein stability. Phosphorylation of S62 by ERK or CDK kinases transiently increases c-Myc stability while phosphorylation of T58 by GSK3β triggers dephosphorylation of S62 by protein phosphatase 2A (PP2A), ubiquitination by the SCF-Fbw7 E3 ligase and proteasomal degradation. Decreased T58 and increased S62 phosphorylation have been observed in human cancer cell lines associated with increased c-Myc protein stability.

使用信息

抗体应用	WB, IF,ELISA,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	57kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Wang X, et al. Cancer Res. 2011, 71(3):925-36.

Application Data

WB

IF