Datasheet

生物描述

特异性	Phospho-EGFR (Y1173) Rabbit Recombinant mAb detects endogenous levels of phosphorylated EGFR (Y1173).
背景	Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that plays key roles in cellular migration, proliferation, and differentiation. The six autophosphorylation sites in the COOH-terminal tail of EGFR are tyrosines 992, 1045, 1068, 1086, 1148, and 1173. Once phosphorylated, these residues serve as docking sites for intracellular signaling proteins containing Src homology 2 (SH2) or phosphotyrosine binding (PTB) domains and provide a connection between the external stimulation and specific internal signal transduction pathways. Phospho-Y992 and -Y1173 recruit and bind to the SH2 domain of phospholipase (PL) Cγ1, resulting in phosphorylation of PLCγ1 on tyrosines 771 and 1254 by EGFR and increased phospholipase activity, which is required for EGF-stimulated cell motility. when phosphorylated, Y1173 serves as a docking site for the adaptor protein Shc, which also increases activity of the MAPK/ERK cascade. Tyr-1173 has also been demonstrated to be binding sites for phosphatases, which play an important role in Tyr dephosphorylation and hence modulation of EGFR activity.

特异性

Phospho-EGFR (Y1173) Rabbit Recombinant mAb detects endogenous levels of phosphorylated EGFR (Y1173).

背景

Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that plays key roles in cellular migration, proliferation, and differentiation. The six autophosphorylation sites in the COOH-terminal tail of EGFR are tyrosines 992, 1045, 1068, 1086, 1148, and 1173. Once phosphorylated, these residues serve as docking sites for intracellular signaling proteins containing Src homology 2 (SH2) or phosphotyrosine binding (PTB) domains and provide a connection between the external stimulation and specific internal signal transduction pathways. Phospho-Y992 and -Y1173 recruit and bind to the SH2 domain of phospholipase (PL) Cγ1, resulting in phosphorylation of PLCγ1 on tyrosines 771 and 1254 by EGFR and increased phospholipase activity, which is required for EGF-stimulated cell motility. when phosphorylated, Y1173 serves as a docking site for the adaptor protein Shc, which also increases activity of the MAPK/ERK cascade. Tyr-1173 has also been demonstrated to be binding sites for phosphatases, which play an important role in Tyr dephosphorylation and hence modulation of EGFR activity.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human

MW (kDa)

175kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck