Phospho-HSF1(S326) Rabbit Recombinant mAb

生物描述

特异性

Phospho-HSF1(S326) Rabbit Recombinant mAb detects endogenous level of total phospho-HSF1(S326).

背景

Heat shock factor 1 (HSF1) monitors the structural integrity of the proteome. Phosphorylation at S326 is a hallmark for HSF1 activation. It is activated in response to stresses such as heat shock, hypoxia, heavy metals, reactive oxygen species, and changes in pH. In an unstressed system, monomeric HSF1 is bound to its negative regulators, heat shock protein 40 (Hsp40), Hsp70, and Hsp90. During stress, HSF1 is released from the complex and undergoes several activating posttranslational modifications that allow it to form a transcriptionally active trimer. In the nucleus, trimeric HSF1 binds to heat shock elements to orchestrate the transcription of large networks of cytoprotective genes, including molecular chaperones, DNA damage repair components, and metabolic enzymes. Activation of HSF1 plays a vital role in human physiology and ageing, as well as in pathological processes such as cardiovascular disease, neurodegeneration, and cancer.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human
MW (kDa)	82kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Dayalan Naidu S, et al. Mol Cell Biol. 2016, 36(18):2403-17.

Application Data

WB

IHC

IF