PKC eta Rabbit Recombinant mAb Datasheet

生物描述

特异性	PKC eta Rabbit Recombinant mAb detects endogenous level of total PKC eta.
背景	Protein kinase C (PKC) is known as a key enzyme in signal transduction, which is involved in the regulation of numerous cellular functions. PKC isoforms comprise a family of serine/threonine kinases, the activity of which is typically initiated by lipid second messengers on the plasma membrane. The phosphorylation of PKC molecules by upstream kinases. PKC eta is predominantly expressed in squamous epithelia or epithelia from which squamous cell carcinomas arise. These include skin, tongue, esophagus, forestomach, trachea and bronchus. In primary human keratinocytes, PKCB is located in the cytoplasm particularly in the perinuclear region, but not in the nucleus. PKC eta is located on the rough endoplasmic reticulum and on the outer nuclear membrane, which is continuous with the endoplasmic reticulum membrane. PKC eta acts as a key regulator in early B-cell development. It plays an important role in the regulation of cell division and cell death during early B-cell development. PKC eta participates in various signal transduction cascades by cross talk with complex signal-transduction networks, such as the RasIMAPK pathway, tyrosine kinase pathways, PKCμ/PKD pathway, and cyclin/cdk pathways.

特异性

PKC eta Rabbit Recombinant mAb detects endogenous level of total PKC eta.

背景

Protein kinase C (PKC) is known as a key enzyme in signal transduction, which is involved in the regulation of numerous cellular functions. PKC isoforms comprise a family of serine/threonine kinases, the activity of which is typically initiated by lipid second messengers on the plasma membrane. The phosphorylation of PKC molecules by upstream kinases. PKC eta is predominantly expressed in squamous epithelia or epithelia from which squamous cell carcinomas arise. These include skin, tongue, esophagus, forestomach, trachea and bronchus. In primary human keratinocytes, PKCB is located in the cytoplasm particularly in the perinuclear region, but not in the nucleus. PKC eta is located on the rough endoplasmic reticulum and on the outer nuclear membrane, which is continuous with the endoplasmic reticulum membrane. PKC eta acts as a key regulator in early B-cell development. It plays an important role in the regulation of cell division and cell death during early B-cell development. PKC eta participates in various signal transduction cascades by cross talk with complex signal-transduction networks, such as the RasIMAPK pathway, tyrosine kinase pathways, PKCμ/PKD pathway, and cyclin/cdk pathways.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

40,78kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Kashiwagi M, et al. J Biochem. 2002, 132(6):853-7.

Application Data

WB

Validated by Selleck