PKR Rabbit Recombinant mAb Datasheet

生物描述

特异性	PKR Rabbit Recombinant mAb detects endogenous level of total PKR.
背景	The protein kinase R (PKR), a ubiquitously expressed serine-threonine kinase, is an intracellular sensor of stress, exemplified by viral infection. Double-stranded (ds) RNA produced during viral replication activates PKR, which in turn arrests protein synthesis by phosphorylating the alpha subunit of the translation initiation factor eIF2. In addition to its translational regulatory function, PKR has a role in signal transduction and transcriptional control through the I B/NF-κB pathway. PKR, which is expressed constitutively in mammalian cells, has also been implicated in the control of cell growth and proliferation with tumor suppressor function. Expression of PKR varies in a timeand tissue-specific manner during human fetal development; levels are imperceptible in blastema and immature mesenchymal cells but high in a variety of more differentiated tissues such as epithelial cells. In adult tissues, PKR levels are low in the proliferating immature zone of squamous mucosa and increase progressively in the nonproliferating mature keratinocytes.

特异性

PKR Rabbit Recombinant mAb detects endogenous level of total PKR.

背景

The protein kinase R (PKR), a ubiquitously expressed serine-threonine kinase, is an intracellular sensor of stress, exemplified by viral infection. Double-stranded (ds) RNA produced during viral replication activates PKR, which in turn arrests protein synthesis by phosphorylating the alpha subunit of the translation initiation factor eIF2. In addition to its translational regulatory function, PKR has a role in signal transduction and transcriptional control through the I B/NF-κB pathway. PKR, which is expressed constitutively in mammalian cells, has also been implicated in the control of cell growth and proliferation with tumor suppressor function. Expression of PKR varies in a timeand tissue-specific manner during human fetal development; levels are imperceptible in blastema and immature mesenchymal cells but high in a variety of more differentiated tissues such as epithelial cells. In adult tissues, PKR levels are low in the proliferating immature zone of squamous mucosa and increase progressively in the nonproliferating mature keratinocytes.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human

MW (kDa)

68kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck