Datasheet

生物描述

特异性	Proteasome 20S LMP2 Rabbit Recombinant mAb detects endogenous levels of total Proteasome 20S LMP2
背景	LMP2 is one of the two proteasome subunits encoded by genes in the major histocompatibility complex class II region. LMP2 mRNA is abundant in lymphoblastoid cell lines, but no detectable amounts are present in brain, smooth muscle, fibroblasts, and colon epithelial cells. LMP2A functions to maintain EBV latency, an effect that is achieved through negative regulation of the BCR. LMP2A blocks BCR signalling by physically excluding the BCR from lipid rafts and by targeting BCR-associated tyrosine kinases (Lyn, Syk) for ubiquitin-mediated degradation. This prevents BCR-mediated intracellular calcium release, protein tyrosine kinase (PTK) signalling, and the switch to lytic replication. LMP2A has been shown to promote β-catenin signalling in epithelial cells, through a mechanism involving phosphorylation and inactivation of glycogen synthase (GSK3), a component of the β-catenin "destruction complex". This ability requires PI3-K/Akt. LMP2A also stimulates mammalian target of rapamycin (mTOR) activity, an effect that is also linked to PI3-K activation. This results in the phosphorylation of p70S6 kinase and the release of elongation initiation factor 4E (eiF4E), which combine to stimulate protein synthesis. LMP2A can inhibit squamous epithelial cell differentiation. In addition, LMP2A can induce an EMT when expressed in certain nasopharyngeal epithelial cell lines.

特异性

Proteasome 20S LMP2 Rabbit Recombinant mAb detects endogenous levels of total Proteasome 20S LMP2

背景

LMP2 is one of the two proteasome subunits encoded by genes in the major histocompatibility complex class II region. LMP2 mRNA is abundant in lymphoblastoid cell lines, but no detectable amounts are present in brain, smooth muscle, fibroblasts, and colon epithelial cells. LMP2A functions to maintain EBV latency, an effect that is achieved through negative regulation of the BCR. LMP2A blocks BCR signalling by physically excluding the BCR from lipid rafts and by targeting BCR-associated tyrosine kinases (Lyn, Syk) for ubiquitin-mediated degradation. This prevents BCR-mediated intracellular calcium release, protein tyrosine kinase (PTK) signalling, and the switch to lytic replication. LMP2A has been shown to promote β-catenin signalling in epithelial cells, through a mechanism involving phosphorylation and inactivation of glycogen synthase (GSK3), a component of the β-catenin "destruction complex". This ability requires PI3-K/Akt. LMP2A also stimulates mammalian target of rapamycin (mTOR) activity, an effect that is also linked to PI3-K activation. This results in the phosphorylation of p70S6 kinase and the release of elongation initiation factor 4E (eiF4E), which combine to stimulate protein synthesis. LMP2A can inhibit squamous epithelial cell differentiation. In addition, LMP2A can induce an EMT when expressed in certain nasopharyngeal epithelial cell lines.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

21kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck