Datasheet

生物描述

特异性	Ras Rabbit Recombinant mAb detects endogenous levels of total Ras.
背景	RAS proteins are binary molecular switches that cycle between active guanosine triphosphate (GTP)-bound and inactive guanosine diphosphate (GDP)-bound states. The RAS family comprises H-Ras, K-Ras 4A, K-Ras 4B, N-Ras, and other homologous proteins such as R-Ras, TC21, Rap, and Ral. Ras proteins are proto-oncogene products that are critical components of signaling pathways leading from cell-surface receptors to the control of cellular proliferation, differentiation, or cell death. Ligand-stimulated activation of the cell-surface receptor, receptor-associated tyrosine kinases, or agonist mediated through G protein–coupled receptors results in the activation of Ras proteins. Activated Ras, in turn, stimulates a cascade of serine/threonine kinases to initiate transcriptional activation of genes. Several proteins with Src homology domains (SH2 and SH3), which mediate protein-protein interaction, have been implicated as connectors of the pathway. RAS effectors are defined as proteins with a strong affinity to GTP–RAS, whose binding is impaired by mutations within the core effector domain. GTP-RAS binds to numerous effectors, such as RAF, PI3K, AF6, to trigger various signaling cascades, which in turn modulate different cellular processes ranging from cell growth, survival, cell migration, differentiation and death.

特异性

Ras Rabbit Recombinant mAb detects endogenous levels of total Ras.

背景

RAS proteins are binary molecular switches that cycle between active guanosine triphosphate (GTP)-bound and inactive guanosine diphosphate (GDP)-bound states. The RAS family comprises H-Ras, K-Ras 4A, K-Ras 4B, N-Ras, and other homologous proteins such as R-Ras, TC21, Rap, and Ral. Ras proteins are proto-oncogene products that are critical components of signaling pathways leading from cell-surface receptors to the control of cellular proliferation, differentiation, or cell death. Ligand-stimulated activation of the cell-surface receptor, receptor-associated tyrosine kinases, or agonist mediated through G protein–coupled receptors results in the activation of Ras proteins. Activated Ras, in turn, stimulates a cascade of serine/threonine kinases to initiate transcriptional activation of genes. Several proteins with Src homology domains (SH2 and SH3), which mediate protein-protein interaction, have been implicated as connectors of the pathway. RAS effectors are defined as proteins with a strong affinity to GTP–RAS, whose binding is impaired by mutations within the core effector domain. GTP-RAS binds to numerous effectors, such as RAF, PI3K, AF6, to trigger various signaling cascades, which in turn modulate different cellular processes ranging from cell growth, survival, cell migration, differentiation and death.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

18kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck