SDHA Rabbit Recombinant mAb Datasheet

生物描述

特异性	SDHA Rabbit Recombinant mAb detects endogenous level of total SDHA.
背景	Succinate dehydrogenase (SDH) is part of both the citric acid cycle and respiratory electron transfer chain. Within the citric acid cycle, SDH oxidizes succinate to fumarate. SDH consists of a hydrophilic head that protrudes into the matrix compartment and a hydrophobic tail that is embedded within the IM with a short segment projecting into the soluble intermembrane space (IMS). The hydrophilic head consists of two subunits forming the catalytic core (Sdh1, Sdh2 in yeast and SdhA, SdhB in mammals) and the membrane domain consists of two subunits (Sdh3, Sdh4 in yeast and SDHC, SDHD in mammals). The catalytic activity of SDH is modulated by post-translational phosphorylation and acetylation as well as active site inhibition. SIRT3 is the major deacetylase controlling the level of Sdh1(SdhA) acetylation. The Sdh1(SdhA) subunit of SDH is phosphorylated in mammalian cells and, like acetylation, this modification appears to attenuate activity.

特异性

SDHA Rabbit Recombinant mAb detects endogenous level of total SDHA.

背景

Succinate dehydrogenase (SDH) is part of both the citric acid cycle and respiratory electron transfer chain. Within the citric acid cycle, SDH oxidizes succinate to fumarate. SDH consists of a hydrophilic head that protrudes into the matrix compartment and a hydrophobic tail that is embedded within the IM with a short segment projecting into the soluble intermembrane space (IMS). The hydrophilic head consists of two subunits forming the catalytic core (Sdh1, Sdh2 in yeast and SdhA, SdhB in mammals) and the membrane domain consists of two subunits (Sdh3, Sdh4 in yeast and SDHC, SDHD in mammals). The catalytic activity of SDH is modulated by post-translational phosphorylation and acetylation as well as active site inhibition. SIRT3 is the major deacetylase controlling the level of Sdh1(SdhA) acetylation. The Sdh1(SdhA) subunit of SDH is phosphorylated in mammalian cells and, like acetylation, this modification appears to attenuate activity.

使用信息

抗体应用

WB, IHC, IF,ELISA,ELISA

稀释比例

WB	IHC	IF
1:1000	1:50	1:50

反应性

Human Mouse Rat

MW (kDa)

72kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Rutter J, et al. Mitochondrion. 2010, 10(4):393-401.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck

IF

Validated by Selleck