SERCA2 Rabbit Recombinant mAb Datasheet

生物描述

特异性	SERCA2 Rabbit Recombinant mAb detects endogenous level of total SERCA2.
背景	Scarco(endo)plasmic Reticulum Calcium-ATPase (SERCA) is a key player in calcium signalling, which is involved in many aspects of cellular function, including transcription, cell motility, apoptosis, exocytosis, and signal transduction. During calcium-mediated signal transduction, the depolarization of the cell membrane in active cells causes an extensive influx of calcium into the cytoplasm. To reduce cytoplasmic Ca2+ concentrations, SERCA uses ATP to actively pump calcium into the sarco(endo)plasmic reticulum for storage. The Ca2+ sequestering activity of SERCA facilitates muscular relaxation in both cardiac and skeletal muscle. The Ca2+ sequestering activity of SERCA is regulated at the level of protein content and is further modified by the endogenous proteins phospholamban (PLN) and sarcolipin (SLN). Additionally, several novel mechanisms, including post-translational modifications and microRNAs (miRNAs) are emerging as integral regulators of Ca2+ transport activity. Of the three mammalian members, SERCA2 is evolutionary the oldest and shows the most wide tissue-expression pattern. Two major SERCA2 splice variants are well-characterized: the muscle-specific isoform SERCA2a and the housekeeping isoform SERCA2b. SERCA2b is found in the ER of most cell types and is considered the housekeeping isoform. The muscle-specific isoform SERCA2a is expressed in the sarcoplasmic reticulum (SR) of the heart and slow-twitch skeletal muscle. Proteins reported to interact with SERCA2 from the cytosolic domain involve the anti-apoptotic Bcl-2, the insulin receptor substrates IRS1/2, the EF-hand Ca2+-binding protein S100A1 and acylphosphatase.

特异性

SERCA2 Rabbit Recombinant mAb detects endogenous level of total SERCA2.

背景

Scarco(endo)plasmic Reticulum Calcium-ATPase (SERCA) is a key player in calcium signalling, which is involved in many aspects of cellular function, including transcription, cell motility, apoptosis, exocytosis, and signal transduction. During calcium-mediated signal transduction, the depolarization of the cell membrane in active cells causes an extensive influx of calcium into the cytoplasm. To reduce cytoplasmic Ca2+ concentrations, SERCA uses ATP to actively pump calcium into the sarco(endo)plasmic reticulum for storage. The Ca2+ sequestering activity of SERCA facilitates muscular relaxation in both cardiac and skeletal muscle. The Ca2+ sequestering activity of SERCA is regulated at the level of protein content and is further modified by the endogenous proteins phospholamban (PLN) and sarcolipin (SLN). Additionally, several novel mechanisms, including post-translational modifications and microRNAs (miRNAs) are emerging as integral regulators of Ca2+ transport activity. Of the three mammalian members, SERCA2 is evolutionary the oldest and shows the most wide tissue-expression pattern. Two major SERCA2 splice variants are well-characterized: the muscle-specific isoform SERCA2a and the housekeeping isoform SERCA2b. SERCA2b is found in the ER of most cell types and is considered the housekeeping isoform. The muscle-specific isoform SERCA2a is expressed in the sarcoplasmic reticulum (SR) of the heart and slow-twitch skeletal muscle. Proteins reported to interact with SERCA2 from the cytosolic domain involve the anti-apoptotic Bcl-2, the insulin receptor substrates IRS1/2, the EF-hand Ca2+-binding protein S100A1 and acylphosphatase.

使用信息

抗体应用

WB, IHC, IF,ELISA,ELISA

稀释比例

WB	IHC	IF
1:1000	1:50	1:50

反应性

Human Mouse Rat

MW (kDa)

110,140kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck

IF

Validated by Selleck