Datasheet

生物描述

特异性	Skp1 Rabbit Recombinant mAb detects endogenous levels of total Skp1
背景	The post-translational modification of degradation of cellular proteins is carried out by E3 ubiquitin ligases. The best-studied E3 complexes are the SCF ligases, which are composed of 3 core components—Cdc53, Skp1, Rbx1—that assemble to the functional ligase complex by binding to one of the multiple substrate adaptors—the F-box proteins. Functioning as adaptors, FBPs bind the Skp1 component of the SCF using a conserved 50 amino acid motif known as the F-box domain (FBD) and recruit post-translationally modified protein substrates to the holoenzyme for ubiquitination via additional protein-protein interaction domains.

特异性

Skp1 Rabbit Recombinant mAb detects endogenous levels of total Skp1

背景

The post-translational modification of degradation of cellular proteins is carried out by E3 ubiquitin ligases. The best-studied E3 complexes are the SCF ligases, which are composed of 3 core components—Cdc53, Skp1, Rbx1—that assemble to the functional ligase complex by binding to one of the multiple substrate adaptors—the F-box proteins. Functioning as adaptors, FBPs bind the Skp1 component of the SCF using a conserved 50 amino acid motif known as the F-box domain (FBD) and recruit post-translationally modified protein substrates to the holoenzyme for ubiquitination via additional protein-protein interaction domains.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

19kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck