Smac/Diablo Rabbit Recombinant mAb

生物描述

特异性

Smac/Diablo Rabbit Recombinant mAb detects endogenous level of total Smac/Diablo.

背景

Smac/DIABLO is a mitochondrial protein that potentiates some forms of apoptosis, possibly by neutralizing one or more members of the IAP family of apoptosis inhibitory proteins. Similar to cytochrome c, Smac/DIABLO is encoded by a nuclear gene and is subsequently imported into mitochondria. During mitochondrial import, the N-terminus of Smac/DIABLO is removed by limited proteolysis to generate the mature form of the molecule. Mature Smac/DIABLO exists as a dimer, mediated by a hydrophobic interface within the N-termini of individual Smac/DIABLO molecules. Mitochondrial Smac/DIABLO release is a general feature of apoptosis, and Bcl-2 regulates this event. Smac/DIABLO efflux from mitochondria is a caspase-catalysed event that occurs downstream of cytochrome c translocation.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	21kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Colin Adrain, et al. EMBO J. 2001, 20(23): 6627–6636.

生物描述

特异性

Smac/Diablo Rabbit Recombinant mAb detects endogenous level of total Smac/Diablo.

背景

Smac/DIABLO is a mitochondrial protein that potentiates some forms of apoptosis, possibly by neutralizing one or more members of the IAP family of apoptosis inhibitory proteins. Similar to cytochrome c, Smac/DIABLO is encoded by a nuclear gene and is subsequently imported into mitochondria. During mitochondrial import, the N-terminus of Smac/DIABLO is removed by limited proteolysis to generate the mature form of the molecule. Mature Smac/DIABLO exists as a dimer, mediated by a hydrophobic interface within the N-termini of individual Smac/DIABLO molecules. Mitochondrial Smac/DIABLO release is a general feature of apoptosis, and Bcl-2 regulates this event. Smac/DIABLO efflux from mitochondria is a caspase-catalysed event that occurs downstream of cytochrome c translocation.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	21kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Colin Adrain, et al. EMBO J. 2001, 20(23): 6627–6636.

Application Data

WB

IHC