Smad1 Rabbit Recombinant mAb Datasheet

生物描述

特异性	Smad1 Rabbit Recombinant mAb detects endogenous level of total Smad1.
背景	Smads are signal transducers for the members of the transforming growth factor-beta (TGF-beta) superfamily. Smads are subdivided into three subclasses based on their structure and function. Receptor-regulated Smads (R-Smads) have a C-terminal Ser-Ser-X-Ser motif, and are directly phosphorylated by the type I receptor kinases. R-Smads then form complexes with common-partner Smads (Co-Smads) and translocate into the nucleus, where they regulate the transcription of target genes. Inhibitory Smads (I-Smads) are induced by ligand stimulation and interfere with the receptor activation or complex formation of R-Smads. Upon BMP or TGFβ stimulation, cytosolic R-Smad members, including Smad1, Smad2, Smad3, Smad5 and Smad8, become phosphorylated by the type I receptors at the most C-terminal SSXS motif. Upon BMP or TGFβ stimulation, cytosolic R-Smad members, including Smad1, Smad2, Smad3, Smad5 and Smad8, become phosphorylated by the type I receptors at the most C-terminal SSXS motif. Although amino acid sequences of R-Smads share extremely high homology, Smad1/5/8 favor BMP signaling, while Smad2/3 mainly act downstream of the TGFβ pathway.

特异性

Smad1 Rabbit Recombinant mAb detects endogenous level of total Smad1.

背景

Smads are signal transducers for the members of the transforming growth factor-beta (TGF-beta) superfamily. Smads are subdivided into three subclasses based on their structure and function. Receptor-regulated Smads (R-Smads) have a C-terminal Ser-Ser-X-Ser motif, and are directly phosphorylated by the type I receptor kinases. R-Smads then form complexes with common-partner Smads (Co-Smads) and translocate into the nucleus, where they regulate the transcription of target genes. Inhibitory Smads (I-Smads) are induced by ligand stimulation and interfere with the receptor activation or complex formation of R-Smads. Upon BMP or TGFβ stimulation, cytosolic R-Smad members, including Smad1, Smad2, Smad3, Smad5 and Smad8, become phosphorylated by the type I receptors at the most C-terminal SSXS motif. Upon BMP or TGFβ stimulation, cytosolic R-Smad members, including Smad1, Smad2, Smad3, Smad5 and Smad8, become phosphorylated by the type I receptors at the most C-terminal SSXS motif. Although amino acid sequences of R-Smads share extremely high homology, Smad1/5/8 favor BMP signaling, while Smad2/3 mainly act downstream of the TGFβ pathway.

使用信息

抗体应用

WB ,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse

MW (kDa)

60kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck