Smad4 Rabbit Recombinant mAb Datasheet

生物描述

特异性	Smad4 Rabbit Recombinant mAb detects endogenous level of total Smad4.
背景	Transforming growth factor-β (TGF-β) family members exert their function via specific type I and type II serine/threonine kinase receptors and intracellular Smad transcription factors, including the common mediator Smad4. Smad4 is the pivotal factor of the TGF-β pathway and functions as a key tumor suppressor. Smad4-mediated cellular responses to TGF-β signaling vary with extracellular matrix, ligand concentration, and cell type specific cofactors at different developmental stages. Upon ligand binding, TGF-βR phosphorylates and activates receptor-associated Smad2 and Smad3, which subsequently associate with a co-Smad, Smad4, to control target gene expression. Smad4 is largely dispensable for T cell activation and Th cell differentiation, it is required for T cell proliferation in response to cognate antigen to promote T-cell-mediated inflammatory disease and tumor rejection.

特异性

Smad4 Rabbit Recombinant mAb detects endogenous level of total Smad4.

背景

Transforming growth factor-β (TGF-β) family members exert their function via specific type I and type II serine/threonine kinase receptors and intracellular Smad transcription factors, including the common mediator Smad4. Smad4 is the pivotal factor of the TGF-β pathway and functions as a key tumor suppressor. Smad4-mediated cellular responses to TGF-β signaling vary with extracellular matrix, ligand concentration, and cell type specific cofactors at different developmental stages. Upon ligand binding, TGF-βR phosphorylates and activates receptor-associated Smad2 and Smad3, which subsequently associate with a co-Smad, Smad4, to control target gene expression. Smad4 is largely dispensable for T cell activation and Th cell differentiation, it is required for T cell proliferation in response to cognate antigen to promote T-cell-mediated inflammatory disease and tumor rejection.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

60kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck