生物描述

特异性

Synaptophysin Rabbit Recombinant mAb detects endogenous levels of total Synaptophysin.

背景

Synaptophysin is an integral membrane glycoprotein that occurs in presynaptic vesicle of neurons and in similar vesicles of the adrenal medulla. It interacts with different synaptic vesicle and motor proteins including v-SNARE vesicle-associated, membrane protein 2/synaptobrevin II (VAMP2), vesicular proton pump V-ATPase, myosin V, dynamin I, and adaptor protein 1 (AP-1). Synaptophysin is involved in different steps of synaptic biogenesis, vesicle protein sorting, vesicle priming, synapse formation, and exo- and endocytosis. It is transported in the fast axonal component in both anterograde and retrograde directions. Antibodies to synaptophysin are used in the identification and characterization of neuroendocrine tumors as a novel type of neurosecretory marker-namely, an integral membrane component, whose expression is not dependent on the synthesis of other markers of neuroendocrine differentiation. Synaptophysin antibodies should also be useful in differential tumor diagnosis, in particular for the exclusion of malignant melanomas, which often present some neuroendocrine markers such as neuron-specific enolase and certain neuropeptides. Synaptophysin is a reliable marker of axonal damage in the CNS in inflammatory/demyelinating conditions.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	38kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Wiedenmann B, et al. Proc Natl Acad Sci U S A. 1986, 83(10):3500-4. [2] Gudi V, et al. J Neuropathol Exp Neurol. 2017, doi: 10.1093/jnen/nlw114.

Application Data

WB

IHC