Tyrosine Hydroxylase Rabbit Recombinant mAb Datasheet

生物描述

特异性	Tyrosine Hydroxylase Rabbit Recombinant mAb detects endogenous level of total Tyrosine Hydroxylase.
背景	Tyrosine hydroxylase (TH) is a rate-limiting step enzyme in the synthesis of catecholamines such as dopamine (DA), epinephrine and norepinephrine (NE). Because of the critical role of catecholamines in maintaining the normal physiological functions, the expression and enzymatic activity of TH are tightly regulated. Particularly for short time changes in neuronal activity, the TH activity will need to modulate fast to meet the local demand. Several post-translational modifications have been shown to regulate TH’s enzymatic activity such as phosphorylation, nitration and S-glutathionylation. While phosphorylation at N-terminal of TH can activate its enzymatic activity, nitration and S-glutathionylation can inactivate TH. The enzyme is inhibited in feedback fashion by the catecholamine neurotransmitters.

特异性

Tyrosine Hydroxylase Rabbit Recombinant mAb detects endogenous level of total Tyrosine Hydroxylase.

背景

Tyrosine hydroxylase (TH) is a rate-limiting step enzyme in the synthesis of catecholamines such as dopamine (DA), epinephrine and norepinephrine (NE). Because of the critical role of catecholamines in maintaining the normal physiological functions, the expression and enzymatic activity of TH are tightly regulated. Particularly for short time changes in neuronal activity, the TH activity will need to modulate fast to meet the local demand. Several post-translational modifications have been shown to regulate TH’s enzymatic activity such as phosphorylation, nitration and S-glutathionylation. While phosphorylation at N-terminal of TH can activate its enzymatic activity, nitration and S-glutathionylation can inactivate TH. The enzyme is inhibited in feedback fashion by the catecholamine neurotransmitters.

使用信息

抗体应用

WB ,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

59kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck