Datasheet

生物描述

特异性	XBP1 Rabbit Recombinant mAb detects endogenous levels of total XBP1.
背景	X-box binding protein 1 (XBP1) is a unique basic region leucine zipper (bZIP) transcription factor whose active form is generated by a nonconventional splicing reaction upon disruption of homeostasis in the endoplasmic reticulum (ER) and activation of the unfolded protein response (UPR). XBP1 is a key regulator of major histocompatibility complex (MHC) class II gene expression in B cells, represents the most conserved signaling component of UPR and is critical for cell fate determination in response to ER stress. Canonical XBP1 targets in UPR signaling include ER chaperones and components of ERAD including ER degradation enhance mannosidase alphalike 1 (EDEM1), DnaJ/Hsp40 homolog subfamily B member 9 (ERDJ4/DNAJB9), and DnaJ/Hsp40 homolog subfamily C member 3 (P58IPK/DNAJC3). Additional tissue-specific XBP1 targets that have been identified include IL-6 in plasma cells, C/EBPα in adipocytes, lipogenic genes in hepatocytes, proinflammatory cytokines in macrophages, and Mist1 in myocytes.

特异性

XBP1 Rabbit Recombinant mAb detects endogenous levels of total XBP1.

背景

X-box binding protein 1 (XBP1) is a unique basic region leucine zipper (bZIP) transcription factor whose active form is generated by a nonconventional splicing reaction upon disruption of homeostasis in the endoplasmic reticulum (ER) and activation of the unfolded protein response (UPR). XBP1 is a key regulator of major histocompatibility complex (MHC) class II gene expression in B cells, represents the most conserved signaling component of UPR and is critical for cell fate determination in response to ER stress. Canonical XBP1 targets in UPR signaling include ER chaperones and components of ERAD including ER degradation enhance mannosidase alphalike 1 (EDEM1), DnaJ/Hsp40 homolog subfamily B member 9 (ERDJ4/DNAJB9), and DnaJ/Hsp40 homolog subfamily C member 3 (P58IPK/DNAJC3). Additional tissue-specific XBP1 targets that have been identified include IL-6 in plasma cells, C/EBPα in adipocytes, lipogenic genes in hepatocytes, proinflammatory cytokines in macrophages, and Mist1 in myocytes.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human

MW (kDa)

29kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Yin He, et al. Gene Expr. 2010, 15(1): 13–25.

Application Data

WB

Validated by Selleck