Berberine chloride
别名: NSC 646666, Natural Yellow 18 chloride 中文名称：盐酸小檗碱，黄连素
Berberine chloride是异喹啉类生物碱的季铵盐。Berberine chloride 可激活 caspase 3 和 caspase 8，分裂 poly ADP-ribose polymerase (PARP) 和释放 cytochrome c。Berberine chloride 可降低 c-IAP1，Bcl-2 和 Bcl-XL 的表达。Berberine chloride 可通过 JNK 和 p38 MAPK 的持续磷酸化以及产生 ROS 来诱导凋亡。Berberine chloride 是一个 topoisomerase I 和 II 的双效抑制剂。Berberine chloride 也是一个潜在的自噬调节剂。
Berberine chloride Chemical Structure
CAS: 633-65-8
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产品质控
Anti-infection抑制剂选择性比较
细胞实验数据示例
| 细胞系 | 实验类型 | 给药浓度 | 孵育时间 | 活性描述 | 文献信息 |
|---|---|---|---|---|---|
| MRC5 cells | Proliferation assay | 10 μM | 54 hrs | Inhibition of HCMV proliferation in MRC5 cells after 54 hrs post-infection at 10 uM by plaque assay | 17239594 |
| MRC5 cells | Proliferation assay | 10 μM | 24 h | Inhibition of HCMV proliferation in MRC5 cells after 24 hrs post-infection at 10 uM by plaque assay | 17239594 |
| HepG2 cells | Function assay | 10 ug/mL | 12 h | Induction of LDLR protein expression in human HepG2 cells at 10 ug/mL after 12 hrs by flow cytometry | 19090767 |
| A10 cells | Function assay | 30 μM | 24 hrs | Downregulation of Scd2 mRNA expression in rat A10 cells at 30 uM after 24 hrs by quantitative RT-PCR analysis | 21401114 |
| A10 cells | Function assay | 30 μM | 24 hrs | Down regulation of Prim2 mRNA expression in rat A10 cells at 30 uM after 24 hrs by quantitative RT-PCR analysis | 21401114 |
| A10 cells | Function assay | 30 μM | 24 hrs | Downregulation of Impk mRNA expression in rat A10 cells at 30 uM after 24 hrs by quantitative RT-PCR analysis | 21401114 |
| HepG2 cells | Function assay | 10 μM | 4 h | Increase in AMPKalpha phosphorylation in human HepG2 cells at 10 uM after 4 hrs by Western blot analysis relative to untreated control | 23058107 |
| HepG2 cells | Function assay | 10 μM | 4 h | Increase in total AMPKalpha level in human HepG2 cells at 10 uM after 4 hrs by Western blot analysis relative to untreated control | 23058107 |
| HepG2 cells | Function assay | 20 μM | 24 hrs | Induction of apoptosis in human HepG2 cells assessed as morphological changes at 20 uM after 24 hrs using Hoechst 33258 staining by fluorescence microscopic analysis | 23182088 |
| HepG2-A16-CD81 cells | Function assay | 10 μM | NOVARTIS: Antimalarial liver stage activity measured as a greater than 50% reduction in Plasmodium yoelii schizont area in HepG2-A16-CD81 cells at 10uM compound concentration, determined by immuno-fluorescence. | 22096101 | |
| HepG2-A16-CD81 cells | Function assay | 10 μM | NOVARTIS: Antimalarial liver stage activity measured as reduction in Plasmodium yoelii schizont area in HepG2-A16-CD81 cells by immuno-fluorescence, and median schizont size at 10uM compound concentration, IC50=0.548 μM | 22096101 | |
| Bel7402 cells | Function assay | 12 h | Induction of LDLR protein in human Bel7402 cells after 12 hrs by RT-PCR assay relative to control | 19090767 | |
| KB cells | Cytotoxicity assay | 72 h | Cytotoxicity against human KB cells after 72 hrs, IC50=7.32 μM | 11141105 | |
| HL60 cells | Apoptosis assay | 48 hrs | Induction of apoptosis in human HL60 cells after 48 hrs using annexin V-propidium iodide staining by FACS analysis | 20227144 | |
| CEM cells | Cytotoxicity assay | 48 hrs | Cytotoxicity against human CEM cells expressing green fluorescent protein after 48 hrs by MTT assay, CC50=2.09 μM | 21295891 | |
| human CEM cells | Function assay | 7 days | Antiviral activity against 0.05 MOI Human immunodeficiency virus 1 NL4.3 infected in human CEM cells expressing green fluorescent protein assessed as p24 antigen production measured 7 days post infection by ELISA, EC50=0.13 μM | 21295891 | |
| SKN cells | Growth inhibition assay | 72 h | Growth inhibition against human SKN cells after 72 hrs by MTT assay, GI50=15.88 μM | 21401114 | |
| RKN cells | Growth inhibition assay | 48 hrs | Growth inhibition against human RKN cells after 48 hrs by MTT assay, GI50=49.6 μM | 21401114 | |
| G402 cells | Growth inhibition assay | 48 hrs | Growth inhibition against human G402 cells after 48 hrs by MTT assay, GI50=11.87 μM | 21401114 | |
| HT-29 cells | Cytotoxicity assay | 48 hrs | Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay, IC50=8.45 μM | 23182088 | |
| HepG2 cells | Cytotoxicity assay | 24 hrs | Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay, IC50=11.22 μM | 23182088 | |
| HepG2 cells | Cytotoxicity assay | 48 hrs | Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay, IC50=8.32 μM | 23182088 | |
| MRC5 cells | Function assay | Antiviral activity against HCMV in MRC5 cells by plaque reduction assay, IC50=0.68 μM | 17239594 | ||
| A549 cells | Cytotoxicity assay | Cytotoxicity against human A549 cells by SRB assay, IC50=6.27 μM | 20594848 | ||
| SKOV3 cells | Cytotoxicity assay | Cytotoxicity against human SKOV3 cells by SRB assay, IC50=16.44 μM | 20594848 | ||
| SK-MEL-2 cells | Cytotoxicity assay | Cytotoxicity against human SK-MEL-2 cells by SRB assay, IC50=13.76 μM | 20594848 | ||
| HCT15 cells | Cytotoxicity assay | Cytotoxicity against human HCT15 cells by SRB assay, IC50=16.59 μM | 20594848 | ||
| 点击查看更多细胞系数据 | |||||
生物活性
| 产品描述 | Berberine chloride是异喹啉类生物碱的季铵盐。Berberine chloride 可激活 caspase 3 和 caspase 8，分裂 poly ADP-ribose polymerase (PARP) 和释放 cytochrome c。Berberine chloride 可降低 c-IAP1，Bcl-2 和 Bcl-XL 的表达。Berberine chloride 可通过 JNK 和 p38 MAPK 的持续磷酸化以及产生 ROS 来诱导凋亡。Berberine chloride 是一个 topoisomerase I 和 II 的双效抑制剂。Berberine chloride 也是一个潜在的自噬调节剂。 | ||||||
|---|---|---|---|---|---|---|---|
| 靶点 |
|
| NCT Number | Recruitment | Conditions | Sponsor/Collaborators | Start Date | Phases |
|---|---|---|---|---|---|
| NCT05845931 | Recruiting | Pharmacokinetic Study in Healthy Volunteers |
University Medicine Greifswald |
May 5 2023 | Not Applicable |
| NCT05480670 | Completed | Polycystic Ovary Syndrome |
Ayub Teaching Hospital |
November 1 2022 | Not Applicable |
| NCT05463003 | Completed | Pharmacokinetic Study in Healthy Volunteers |
University Medicine Greifswald |
July 19 2022 | Not Applicable |
化学信息&溶解度
| 分子量 | 371.81 | 分子式 | C20H18NO4.Cl |
| CAS号 | 633-65-8 | SDF | Download Berberine chloride SDF |
| 储存条件(自收到货起) | |||
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体外溶解度 |
DMSO : 25 mg/mL ( (67.23 mM)； DMSO吸湿会降低化合物溶解度，请使用新开封DMSO) Water : Insoluble Ethanol : Insoluble |
摩尔浓度计算器 |
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体内溶解度 现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂 |
动物体内配方计算器 | ||||
实验计算
动物体内配方计算器（澄清溶液）
第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）
第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）
计算结果：
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；
体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH2O，混匀澄清。
体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。
注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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