Gefitinib (ZD1839)

目录号:S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD1839)是一种EGFR抑制剂,作用于NR6wtEGFR和NR6W细胞中的Tyr1173,Tyr992,Tyr1173和Tyr992,IC50 分别为37 nM,37nM,26 nM和57 nM。

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客户购买Selleck的此次产品后发表的文献92篇:

客户使用该产品的13个实验数据:

  • Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

    Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

  • (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

    LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

  • Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of 5-HT-induced 5-HT3A and 5-HT3AB receptor currents.

    Eur J Pharmacol, 2017, 821:21-28. Gefitinib (ZD1839) purchased from Selleck.

    Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

    Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

  • MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

产品安全说明书

EGFR抑制剂选择性比较

生物活性

产品描述 Gefitinib (ZD1839)是一种EGFR抑制剂,作用于NR6wtEGFR和NR6W细胞中的Tyr1173,Tyr992,Tyr1173和Tyr992,IC50 分别为37 nM,37nM,26 nM和57 nM。
特性 Gefitinib是有效的EGFR酪氨酸激酶抑制剂。
靶点
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
体外研究

Gefitinib 作用于高和低表达EGFR的细胞系,包括NR6, NR6M 和 NR6W 细胞系,有效抑制EGFR上全部酪氨酸磷酸化位点。磷酸化位点 Tyr1173和Tyr992敏感性较低,需要高浓度Gefitinib才能抑制。Gefitinib作用于NR6W细胞,高效阻断PLC-γ磷酸化, IC50为27nM。NR6wtEGFR 和NR6M细胞系具有低水平PLC-γ磷酸化, IC50 分别为 43 nM和 369 nM。Gefitinib抑制AKT磷酸化,作用于低EGFR和EGFRvIII表达的细胞系, IC50 分别为220 和263nM。Gefitinib按 0.1 到 0.5μM剂量处理,显著促进而不是废除 NR6M细胞形成集落。然而, 2μM Gefitinib 完全抑制 NR6M 集落形成。Gefitinib 作用于高和低EGFR表达的细胞系,EGF 刺激72小时后,快速抑制EGFR 和ERK 磷酸化,这种作用存在剂量依赖性。[1]Gefitinib抑制 EGF驱动的未转化MCF10A 细胞生长,IC50为 20 nM。[2] Gefitinib 作用于MDA-MB-231细胞,抑制EGF信号。Gefitinib严重抑制细胞数的增多,这种作用存在剂量依赖性。 [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB MkPLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTBwMEW0JO69VQ>? Mn\6NVY2OTZ2N{O=
NIH3T3 NWKzXFJPTnWwY4Tpc44hSXO|YYm= M1q2XlAvOiEQvF2= M4T4NFIhcA>? NXjDUYhZTE2VTx?= NYnDfIpCUW6qaXLpeIlwdiCxZjDFS2Yhe3SrbYXsZZRm\CCqdX3hckBmemKEMTDheZRweGixc4Doc5J6dGG2aX;uJJdqfGhiSVO1NEBw\iByLkCxOFQh|ryP NXjSeGNnOTZ2OECyPFQ>
COS7 MnvaSpVv[3Srb36gRZN{[Xl? NHjTZ4Y6PiEQvF2= MlvpTY5pcWKrdHnvckBw\iCqdX3hckBLSUt{IG[2NVdHKG23dHHueEBmgHC{ZYPz[YQ> NWO5U3BkOTdzN{i3NlI>
A549 NISwVnhEgXSxdH;4bYMhSXO|YYm= MVO5OkBp NH3qcI1FVVOR M3LzeWlEPTB;MUOuOVkh|ryP MlLvNVg{OTN6MEe=
K562 NXyydGVSS3m2b4TvfIlkKEG|c3H5 M2L5Olk3KGh? NYrueW13TE2VTx?= MnrLTWM2OD17LkO2JO69VQ>? NW\pRWpoOTh|MUO4NFc>
MDA-MB-231 NYi0coQ4S3m2b4TvfIlkKEG|c3H5 MlPKPVYhcA>? MVXEUXNQ M4HtfmlEPTB;M{[uOlEh|ryP NGHlOHgyQDNzM{iwOy=>
MOLT4 NHfZNZFEgXSxdH;4bYMhSXO|YYm= NXLDWlVSQTZiaB?= NXXwWFVnTE2VTx?= MYrJR|UxRTF3LkCyJO69VQ>? M3rnclE5OzF|OEC3
MCF7 NIL2elBEgXSxdH;4bYMhSXO|YYm= M{TCU|UxKM7:TR?= NHPBW2Y4OiCq MnzPTWM2OD1zMj6wOUDPxE1? M33GVlE5PzdzOEG5
BT474 Mn;uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NELh[mw4OiCq MX;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEKWNEe0JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWUQnKyJJdqfGhiRVO1NEBw\iByLkOg{txO MYmxPVAzQDR{NB?=
HN5 M2\a[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV\jWVJ{PzJiaB?= M3jGNmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSF61JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKge4l1cCCHQ{WwJI9nKDBwMEGg{txO MkfjNVkxOjh2MkS=
BA/F3 M1\RSGN6fG:2b4jpZ{BCe3OjeR?= Ml\KO|IhcA>? NVnVNlRXS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhSkFxRkOgZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKEx6NUjSJI12fGGwdDD3bZRpKEmFNUCgc4YhOC5yMUC4JO69VQ>? NXfqWm5EOTl{M{myNlk>
NIH3T3 M37VTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLXPY1DPzJiaB?= MUXHdo94fGhiaX7obYJqfGmxbjDv[kBud3W|ZTDOTWgwO1R|IHPlcIx{KGW6cILld5NqdmdiZYLCNkBo\W6nIIfpeIghUUN3MDDv[kAxNjV2IN88US=> M{n5dlE6PjZ3M{e3
MCF7 MX MnO2SpVv[3Srb36gRZN{[Xl? NWn4NHo3OzBibXnu M{LCV2lvcGmkaYTpc44hd2ZiQlPSVEBmgHC{ZYPz[YQhf2m2aDDJR|UxKG:oIEGuNUDPxE1? NWrhfppHOTl7M{K5OlA>
A431 MorxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTYXHpJOTBizszN M2TWZlczKGh? M3Hs[mdKPTB;MD61NVQh|ryP NIrvUWgyQTl4OUS2OS=>
A431 NF;scWNMcW6jc3WgRZN{[Xl? NYnHN2Z4OC5zIN88US=> M3f4OlEhcA>? MojRTY5pcWKrdHnvckBw\iCHR1[tbY5lfWOnZDDFS2ZTKHCqb4PwbI9zgWyjdHnvci=> MoT5NVk6Pjl2NkW=
A431 MVPLbY5ie2ViQYPzZZk> M3\VbFExOCEQvF2= M1PtW|ExKG2rbh?= NFXaVFZRem:ub37n[YQhcW6qaXLpeI9zgSCjY4Tpeol1gSCxZjDFS2YucW6mdXPl[EBGT0[UIIDoc5NxcG:{eXzheIlwdg>? NVy3fXkyOTl7Nkm0OlU>
Sf9 NISwN3lMcW6jc3WgRZN{[Xl? M4r3TFExKG2rbh?= NFr6PWdFVVOR NFjr[nBKdmirYnn0bY9vKG:oIFXHSnIh\XiycnXzd4VlKGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhemWlZYD0c5Ih[XW2b4Doc5NxcG:{eXzheIlwdiC5aYToJGlEPTBib3[gNE4xOzNizszN NGP6PXkzODB3NkSyOS=>
NCI-H1975 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnHiOUDPxE1? MkfzO|IhcA>? NXrL[Ih{UUN3ME24MlI3KM7:TR?= MlvaNlAyPTF4N{C=
NCI-H1975 NUX5OGo4U2mwYYPlJGF{e2G7 M4jEeVExKM7:TR?= MknNTY5pcWKrdHnvckBw\iCHR1\SJIF2fG:yaH;zdIhwenmuYYTpc44> NGfkfmYzODF3MU[3NC=>
NCI-H1975 MXjGeY5kfGmxbjDBd5NigQ>? M1G5ZlExKM7:TR?= NIPvVIVKdmirYnn0bY9vKG:oIFXSRmIzKGG3dH;wbI9{eGixconsZZRqd25? NHXaclEzODF3MU[3NC=>
NCI-H1975 MlPDSpVv[3Srb36gRZN{[Xl? M{DXXlUh|ryP M1O1PVQ5KGh? NHztVnlKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHHzd4V{e2WmIHHzJIFkfGm4YYTpc44hd2ZiY3HzdIF{\S1| M33BeVIxOTVzNkew
A375 NX:yc2J5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHL2cGs1QCCq MoLuTWM2OD1{MD61OUDPxE1? MYiyNFMxPDV|Nx?=
SMMC7721 NEDvXGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXK0PEBp M1S3NWlEPTB;M{OuNVIh|ryP MoXSNlA{ODR3M{e=
DU145 M37NO2N6fG:2b4jpZ{BCe3OjeR?= MoHPOUBl NGnwc4NFVVOR NWroTo9[UUN3ME20Nk4yPyEQvF2= M4fqVVIxPDZ4NUW1
MIAPaCa2 M4DsXWN6fG:2b4jpZ{BCe3OjeR?= Mlu5OUBl MkPCSG1UVw>? M{O4bmlEPTB;NUCuOlIh|ryP NGjrTmgzODR4NkW1OS=>
PANC1 MVnDfZRwfG:6aXOgRZN{[Xl? MmXyOUBl NXzoXIE4TE2VTx?= M3LTUmlEPTB;NECuOFIh|ryP NGrxSYgzODR4NkW1OS=>
PC9 NEjnNpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFXHSnIu\GWoaXPp[Y51KGi3bXHuJHBEQSClZXzsd{B4cXSqIFXDOVAhd2ZiMD6wNVch|ryP NWe0cVlGOjF{MEi4NFI>
PC9 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3rVVWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[5Jwf3SqLYLld4l{fGGwdDDoeY1idiCSQ{mgZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKEV5NE\fRVc2OC:WN{mwUUBufXSjboSge4l1cCCHQ{WwJI9nKDdizszN NHPsO2kzOTJyOEiwNi=>
PC3 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXyxNEDPxE1? NHqxTGg4OiCq NFP1N3JFVVOR M1TkNWlEPTB;Nz60JO69VQ>? NYj3R|VsOjF|NUO1OFY>
MDA-MB-468 M{jv[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETR[IMyODBizszN MkTkOFghcA>? MWfEUXNQ MW\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eR?= M13j[FIyPDJ7NkOy
Plasmodium falciparum NXjQNXZ[eUiWUzDwdo9ncWyrbneg[o9zKGmwaHnibZRwenNib3[gVIxie22xZHn1cUBn[WylaYDhdpVuKHC{b3zp[oVz[XSrb36= MnnpNlE5OTdyNEW=
NCI-H1993 NXfNcoVtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofCO|IhcA>? NXvPR5VRTE2VTx?= M165N2lEPTB;MD6xJO69VQ>? NVrNOIp2OjF7MU[0N|M>
NCI-H292 MmLhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjuO|IhcA>? NYO0[5cxTE2VTx?= MmrITWM2OD1yLkCyJO69VQ>? MXuyNVkyPjR|Mx?=
A431 MWHLbY5ie2ViQYPzZZk> MmrqNkBp MYDEUXNQ MWjJcohq[mm2aX;uJI9nKEWJRmKgdIhwe3Cqb4L5cIF1cW:wIHnuJGVITi2|dHnteYxifGWmIHj1cYFvKEF2M{Gge4l1cCCLQ{WwJI9nKDBwMECxJO69VQ>? MWqyNlExOTF|Mh?=
MCF7 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlWwNVAxyqEQvHevcWw> NIX2TVc1QCCq M133TWROW09? NEnS[IhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgc5ZmemW6cILld5NqdmdiRVfGVkBo\W6nIIfpeIghUUN3MDDv[kAyOi5yNTFOwG0> Mle0NlIyOTlzM{C=
BT474 NVzpUY5mS3m2b4TvfIlkKEG|c3H5 M17rflExKM7:TR?= M1nqPFQ5KGh? MYDDfZRwfG:6aXPpeJkh[WejaX7zeEB{\XK3bT3zeIFzfmWmIHj1cYFvKEKWNEe0JINmdGy| Mn\vNlIyOzh|MEi=
MCF7 NUK4dGR{S3m2b4TvfIlkKEG|c3H5 MkHaNVAh|ryP NUPib4NwPDhiaB?= MnO3R5l1d3SxeHnjbZR6KGGpYXnud5Qhe2W{dX2td5Rien[nZDDoeY1idiCPQ1[3JINmdGy|IH;2[ZIu\XiycnXzd4lv\yCHcnLCNkBk\Wyucx?= NX3RNmhoOjJzM{izNFg>
HCC827 MoLaT4lv[XOnIFHzd4F6 NXnTUnZ1OC5zMkWg{txO NXvve|dwOjRiaB?= NFXIdnBFVVOR NEHEVo1KdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRiWUGwOlgh[XW2b4Doc5NxcG:{eXzheIlwdg>? NV\ueo5MOjJ|M{mzOFI>
HCC827 M3u1SGtqdmG|ZTDBd5NigQ>? MWmwMlEzPSEQvF2= MkTHNlQhcA>? MX3EUXNQ MVzJcohq[mm2aX;uJI9nKEWJRmKg[IVtKEV5NE[tRVc2OCCvdYThcpQudWWmaXH0[YQhTXKtIIDoc5NxcG:{eXzheIlwdg>? M3T4bVIzOzN7M{Sy
HCC827 NFTLfmNMcW6jc3WgRZN{[Xl? MYSwMlEzPSEQvF2= NXThUZZHOjRiaB?= Ml;qSG1UVw>? NWXUfZFzUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62LX3l[IlifGWmIFHreEBxcG:|cHjvdplt[XSrb36= M1XlbVIzOzN7M{Sy
A431 NXvaNoQ6U2mwYYPlJGF{e2G7 NETmcGEyODBizszN MlfzN|AhdWmw NHqxT3FKdmirYnn0bY9vKG:oIFXHSnIh\GW{aY\l[EBnem:vIHj1cYFvKEF2M{GgZ4VtdCCvZX3idoFv\SCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHCxbImtS4x2NVS7cjDwbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFEvODhizszN Mn3iNlI3QTh5OEK=
H460 MoryR5l1d3SxeHnjJGF{e2G7 Moe1O|IhcA>? MoLPTWM2OD13LkW5JO69VQ>? NWXFT3ROOjJ5Mke0OFk>
HT-29 MUPDfZRwfG:6aXOgRZN{[Xl? MnfvO|IhcA>? MkCxTWM2OD1|LkO2JO69VQ>? MorRNlI4Ojd2NEm=
SGC7901 NW[0UpF{S3m2b4TvfIlkKEG|c3H5 MYW3NkBp M4T0T2lEPTB;MUCuNlYh|ryP MonMNlI4Ojd2NEm=
A498 NWDmPVhST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF75OndCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG0PVgh[2WubIOg[ZhxemW|c3nu[{BGT0[UIHHu[EBUWkNid3n0bEBKSzVyIH;mJFgvPiEQvF2= NGn0ZYszOjhzOEi0PC=>
Calu3 MV3DfZRwfG:6aXOgRZN{[Xl? NHjHUWgyOCEQvHevcWw> NUD2TJRjPzJiaB?= M2nERWlEPTB;MT62JO69VQ>? NEjFZmszOzFzNkG2PC=>
FADU MUnDfZRwfG:6aXOgRZN{[Xl? MmjzNVAh|rypL33M NFLJZXI4OiCq NEP0fYdKSzVyPUCuNFUh|ryP NVSxPFZ{OjNzMU[xOlg>
HCC827 MUXDfZRwfG:6aXOgRZN{[Xl? M{S1b|ExKM7:Zz;tUC=> M4\CTVczKGh? MUnJR|UxRTBwMECxOUDPxE1? NWnBOY1YOjNzMU[xOlg>
PC9 MkTRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvWeJgyOCEQvHevcWw> NF;CTHA4OiCq MYrJR|UxRTBwMECxOEDPxE1? MUCyN|EyPjF4OB?=
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BT-20 M3X1Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFnocYtKSzVyPUKuNlY4PDRizszN MmnUV2FPT0WU
A498 MlXMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTJwMkm0NVUh|ryP NXWxbpptW0GQR1XS
IGROV-1 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHoRolrUUN3ME2yMlMyOjN5IN88US=> MVzTRW5ITVJ?
JVM-3 NHGy[VlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2L4T2lEPTB;Mj6zOFY1OyEQvF2= Ml7TV2FPT0WU
NCI-H2170 NFm5RVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M321XGlEPTB;Mj6zOFc5OyEQvF2= NVrwVnpLW0GQR1XS
EW-18 NIH1NHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkLzTWM2OD1{LkO1OFcyKM7:TR?= MXzTRW5ITVJ?
KU812 MofXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjPTWM2OD1{LkO2O|c6KM7:TR?= MYDTRW5ITVJ?
NY MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPRTFJKSzVyPUKuN|czPyEQvF2= NIC5ZVBUSU6JRWK=
HTC-C3 M1T2W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmG0TWM2OD1{LkSyNlY4KM7:TR?= MlLsV2FPT0WU
CFPAC-1 M1L6Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTJwNEO4OlQh|ryP NWXaXZAzW0GQR1XS
HH NX6xeohnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{Hh[2lEPTB;Mj60PVg2PCEQvF2= NF:5fm1USU6JRWK=
MKN7 M1Ox[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{nHXGlEPTB;Mj62Nlc1QCEQvF2= M{fNbXNCVkeHUh?=
TE-8 M4LzS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mlv3TWM2OD1{Lk[zOlU{KM7:TR?= NXnKZmE5W0GQR1XS
MC-IXC M3;aVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MofXTWM2OD1{Lk[0OFU{KM7:TR?= MnXYV2FPT0WU
DMS-79 MlrmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYTJR|UxRTJwNkmyPFQh|ryP MX7TRW5ITVJ?
Detroit562 NX3LcZRqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojCTWM2OD1{Lk[5PFQ2KM7:TR?= M2iwd3NCVkeHUh?=
HuP-T4 NGj3OWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fnWmlEPTB;Mj63NlgyQCEQvF2= NY\RZW44W0GQR1XS
QIMR-WIL MkXNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1ywc2lEPTB;Mj63OVk2OiEQvF2= MoXYV2FPT0WU
BB65-RCC MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\kflhMUUN3ME2yMlgxPzF|IN88US=> MVvTRW5ITVJ?
PC-3 NG\EN3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDGTWM2OD1{LkizN|E4KM7:TR?= MX7TRW5ITVJ?
OVCAR-3 NIT6bm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI[1XVNKSzVyPUKuPFU2QTRizszN NHPWb3dUSU6JRWK=
KYSE-510 MkP3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRTJwOE[3PFYh|ryP M1W0VHNCVkeHUh?=
HC-1 NHToW3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTJwOUSwNVIh|ryP NXjaSZVXW0GQR1XS
NCI-H1693 NUnCSZQ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTJwOUW4PVch|ryP MYrTRW5ITVJ?
DU-4475 NHK4V2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWfVfZd{UUN3ME2yMlk6ODV{IN88US=> MmHBV2FPT0WU
COLO-680N M3TFe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXYTWM2OD1|LkCwOFk2KM7:TR?= MmHyV2FPT0WU
MN-60 NFH6b3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXKTWM2OD1|LkC0PVc1KM7:TR?= NVy0copLW0GQR1XS
BCPAP MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7uSlJSUUN3ME2zMlA5Ojl3IN88US=> M{jINXNCVkeHUh?=
647-V M4jqeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmS1TWM2OD1|LkG2NlIh|ryP MmTZV2FPT0WU
HT-1376 M2XQXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTNwMkC1NFYh|ryP Mnv2V2FPT0WU
NCI-H1793 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLoTWM2OD1|LkKxNFk5KM7:TR?= Mlu0V2FPT0WU
SW1463 MnXOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVLCdJpSUUN3ME2zMlI3OTl{IN88US=> MV7TRW5ITVJ?
NCI-H727 NGrCcpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvpeG5KSzVyPUOuN|g2QDRizszN MoX1V2FPT0WU
LB771-HNC NEeyXmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoXITWM2OD1|LkO5NVI3KM7:TR?= NYfye4YyW0GQR1XS
D-542MG MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3njU2lEPTB;Mz60OFIzOiEQvF2= NXzv[I06W0GQR1XS
NTERA-S-cl-D1 M3rEWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFX2VFBKSzVyPUOuOFQ6PTdizszN MnzRV2FPT0WU
C8166 M4DNZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVzaPYNsUUN3ME2zMlQ3PTJzIN88US=> M3\RO3NCVkeHUh?=
639-V MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTNwNUe3PVIh|ryP M1TNZXNCVkeHUh?=
TE-1 MnX4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPkXZljUUN3ME2zMlU6OTZ7IN88US=> MXXTRW5ITVJ?
OAW-28 Ml3ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3K1Z2lEPTB;Mz61PVQzPiEQvF2= NEHYVmpUSU6JRWK=
SK-LU-1 M2LBWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYr4UZBJUUN3ME2zMlYxPjR3IN88US=> MWPTRW5ITVJ?
CaR-1 MnXVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1fzNWlEPTB;Mz62NVA1QCEQvF2= NHvLcWJUSU6JRWK=
A2780 MoLyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTNwNkSwPFkh|ryP MorkV2FPT0WU
C2BBe1 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\ZN3NDUUN3ME2zMlY1PDB5IN88US=> NXvUNmFqW0GQR1XS
SK-MES-1 M{fzfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrKOZl5UUN3ME2zMlY1PjF2IN88US=> Ml2wV2FPT0WU
PANC-10-05 NGDvPXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTNwN{KwO|Ih|ryP NIrPc2ZUSU6JRWK=
J-RT3-T3-5 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxRTNwN{W0NFYh|ryP M{LMXHNCVkeHUh?=
ES4 NWjGPYZTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIW5TWNKSzVyPUOuPFIzQDZizszN NXr3eHJYW0GQR1XS
NCI-H28 NUn5[VNLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLydog6UUN3ME2zMlgzQTZzIN88US=> NX3ybYZiW0GQR1XS
NCI-H1155 MlfSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFnVXWJKSzVyPUOuPFQyPTlizszN Mnr2V2FPT0WU
KURAMOCHI NWHMOpVuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLmVmJKSzVyPUOuPFYxPTZizszN MWLTRW5ITVJ?
NCI-H69 NHfRZpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVzCVI8xUUN3ME2zMlkxOTd6IN88US=> MVvTRW5ITVJ?
IST-MES1 Ml3JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoL5TWM2OD1|Lkm0NFI6KM7:TR?= NXrWSWhOW0GQR1XS
NH-12 NVf3Xop{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV7JR|UxRTNwOUSxPFch|ryP MXTTRW5ITVJ?
JVM-2 NGnteJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoflTWM2OD1|Lkm0O|UzKM7:TR?= M2HiPHNCVkeHUh?=
EW-16 Mme2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3QTWM2OD1|Lkm1OVc4KM7:TR?= M13E[3NCVkeHUh?=
NCI-H441 M2[zTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvBZ3dtUUN3ME2zMlk4ODF2IN88US=> MYHTRW5ITVJ?
RCC10RGB NGfFbJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTRwMEGyOFYh|ryP NHf6ZmtUSU6JRWK=
TCCSUP MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzhTWM2OD12LkGxNFUh|ryP MUjTRW5ITVJ?
HL-60 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTRwMUKyOFEh|ryP NVj6d2R7W0GQR1XS
EW-11 M1XtSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH72cphKSzVyPUSuNVMzPjFizszN NI\heIpUSU6JRWK=
KARPAS-299 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PPeGlEPTB;ND6zNVAyOyEQvF2= MVrTRW5ITVJ?
SNU-C2B NUDUU|Q{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIXiUHlKSzVyPUSuN|Q3QDZizszN NFXNN4FUSU6JRWK=
LU-139 NF65NnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTRwM{eyOFch|ryP MWjTRW5ITVJ?
HCC1937 M2nEUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjxTWM2OD12LkO3PVk{KM7:TR?= M3vndHNCVkeHUh?=
GR-ST NH\PS4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXjSnpKSzVyPUSuOFMyQCEQvF2= NFHsRYFUSU6JRWK=
SK-OV-3 M2XCNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrLbFlwUUN3ME20MlQ3ODR{IN88US=> M33ZRXNCVkeHUh?=
A172 M{TJfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjCb4RWUUN3ME20MlU2PTJzIN88US=> NYX2SFNjW0GQR1XS
NB10 MmjIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTRwNUm3PFEh|ryP MmHGV2FPT0WU
P30-OHK MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3nyXWlEPTB;ND64NVkzQSEQvF2= NVToe5luW0GQR1XS
KYSE-520 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUCzTWV1UUN3ME20Mlg5ODF6IN88US=> M2LvfHNCVkeHUh?=
KNS-62 NVvoeXV[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkXYTWM2OD12LkmwPFA4KM7:TR?= MYPTRW5ITVJ?
ES1 MkDPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTRwOU[wN|Uh|ryP MkjXV2FPT0WU
KOSC-2 M1jTTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTRwOUm5OVkh|ryP NUXOZndQW0GQR1XS
TE-9 NGPne3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDDTWM2OD13LkCxOVI2KM7:TR?= MXjTRW5ITVJ?
NCI-H1838 Mo\HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEC4SHFKSzVyPUWuNFIxPzZizszN MX3TRW5ITVJ?
KP-N-YN M37ZPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFjBUlZKSzVyPUWuNVI2QTFizszN MnPDV2FPT0WU
ALL-PO NHLIc21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknjTWM2OD13LkKyOVg4KM7:TR?= M1fjOHNCVkeHUh?=
NOMO-1 NGGwUFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkPOTWM2OD13LkKzNVc3KM7:TR?= NVfCS|ZpW0GQR1XS
CGTH-W-1 NHLzd3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7n[ZNkUUN3ME21MlM5PTh2IN88US=> NWDaTnNWW0GQR1XS
ATN-1 NIHEclhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\RTWM2OD13LkSxNVg1KM7:TR?= NXXic25{W0GQR1XS
AGS MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlv4TWM2OD13LkS5OlE5KM7:TR?= MXnTRW5ITVJ?
SW13 Ml;5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHXTWM2OD13LkWzNlY2KM7:TR?= M{WzZ3NCVkeHUh?=
SW1990 Moq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFPpTolKSzVyPUWuOlIzQDVizszN MYjTRW5ITVJ?
BFTC-905 M3vDOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHlNXlKSzVyPUWuOlU5PzhizszN NGHLXoVUSU6JRWK=
RMG-I MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlrFTWM2OD13Lk[1PVQzKM7:TR?= NWjNbGdDW0GQR1XS
HD-MY-Z MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TNWWlEPTB;NT62PFU5OSEQvF2= M2jkU3NCVkeHUh?=
HCC1187 M2HiTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTVwN{GwN|Ih|ryP M4\vRnNCVkeHUh?=
HCC2998 NE\4W5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHXTWM2OD13LkexPFc4KM7:TR?= NYjNV5JKW0GQR1XS
RT-112 Ml\OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIryOnFKSzVyPUWuPVAzPDVizszN MUHTRW5ITVJ?
CAKI-1 M2jrZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTZwMEC5PFUh|ryP MYHTRW5ITVJ?
HDLM-2 NF;rdW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{PpSGlEPTB;Nj6wNVQ5QSEQvF2= NYXtO41vW0GQR1XS
TE-10 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2LvXGlEPTB;Nj6wOFg{OyEQvF2= NU\LVmhqW0GQR1XS
KE-37 MlzLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPrNXpKSzVyPU[uNFczPThizszN NYXOOldoW0GQR1XS
RCM-1 NIjZSZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1jGN2lEPTB;Nj6wO|cyQSEQvF2= NY\qcVc2W0GQR1XS
Mewo M{\CSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkDpTWM2OD14LkGxOFg4KM7:TR?= M4\oeXNCVkeHUh?=
L-428 M{f2bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{P1TWlEPTB;Nj6xPFA{PyEQvF2= M2rtb3NCVkeHUh?=
8-MG-BA M1vhSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTZwMUmzO|kh|ryP NGq0XZRUSU6JRWK=
SK-MEL-28 MlHXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1PaVWlEPTB;Nj6yNFk3PyEQvF2= MlTwV2FPT0WU
VA-ES-BJ Mon6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWfJR|UxRTZwM{C0OlIh|ryP NIOxNYdUSU6JRWK=
CAL-120 M4HrOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{PSOGlEPTB;Nj60OVE3OiEQvF2= MmLKV2FPT0WU
GAMG NWjpOIxtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlniTWM2OD14LkWwOlIh|ryP MYrTRW5ITVJ?
MSTO-221H M4TVe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVzRUmFiUUN3ME22MlUyOTdizszN NXS2VWY2W0GQR1XS
GCT NE\3fGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1LrZmlEPTB;Nj61N|M5PyEQvF2= NID5XZdUSU6JRWK=
SH-4 NXvmfZJYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\EN2JKSzVyPU[uOVg5PDRizszN MUjTRW5ITVJ?
Calu-3 NHvHfWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHpTWM2OD14Lk[1OVY5KM7:TR?= NHXjPJFUSU6JRWK=
SK-HEP-1 MnjiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\rO2lEPTB;Nj63NVc2OyEQvF2= M2KxeHNCVkeHUh?=
PFSK-1 MnrSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3zleWlEPTB;Nj63N|Q4QCEQvF2= MoDPV2FPT0WU
NB12 NWq5ZWg6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7HOJFKSzVyPU[uO|g1ODRizszN M37kdXNCVkeHUh?=
A388 M4XlUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{nZXWlEPTB;Nj64PVUzKM7:TR?= M2nV[nNCVkeHUh?=
KLE MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHsTWM2OD14Lkm2OVE3KM7:TR?= M4rITXNCVkeHUh?=
TE-11 Mlr6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTdwMES5Olch|ryP MnT6V2FPT0WU
NCI-H2405 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1[2R2lEPTB;Nz6wPFM6OyEQvF2= M4HocXNCVkeHUh?=
OVCAR-4 M3LscWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmHQTWM2OD15LkG1N|Uh|ryP NGrHb2ZUSU6JRWK=
KP-N-YS MoThS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHkXmhSUUN3ME23MlE5OTRizszN M2XCenNCVkeHUh?=
NOS-1 MkPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1XLeGlEPTB;Nz6yOVgzQCEQvF2= MWLTRW5ITVJ?
DMS-114 M1L5eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmfnTWM2OD15LkK5OlMyKM7:TR?= MXrTRW5ITVJ?
NCI-H2030 NILvdZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlexTWM2OD15LkOzOlk5KM7:TR?= NX7qTVV4W0GQR1XS
HEL MnP1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XFUWlEPTB;Nz6zO|UzOSEQvF2= NUfHb3B[W0GQR1XS
CAL-62 MnvYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7WTlFkUUN3ME23MlM6QDZ4IN88US=> MYLTRW5ITVJ?
COLO-668 M1znbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX7yfnB1UUN3ME23MlQ{QTZ5IN88US=> NH;uZ5BUSU6JRWK=
SKG-IIIa Mk\2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTdwNEWzPFEh|ryP M{fNNnNCVkeHUh?=
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ONS-76 NEX1OnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|UxRTN5LkGzNFkh|ryP MV;TRW5ITVJ?
T98G MnnpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmHGTWM2OD1|Nz64OlM2KM7:TR?= M{C0WHNCVkeHUh?=
NB14 NVT0VWh{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTN6LkS1OFQh|ryP M{i4c3NCVkeHUh?=
D-423MG NGPEd3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF7sfVBKSzVyPUO4MlQ5PjlizszN MnrlV2FPT0WU
NCI-H510A M4PGb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnywTWM2OD1|OD62OFUyKM7:TR?= MUjTRW5ITVJ?
NMC-G1 MoLZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DNbGlEPTB;M{iuO|IzOiEQvF2= NF[1dJNUSU6JRWK=
SW948 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PFfmlEPTB;M{iuPFk1QSEQvF2= MWXTRW5ITVJ?
MFM-223 NIDGXlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEPBPZJKSzVyPUO4Mlk1OzFizszN MV3TRW5ITVJ?
PAN1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLUXXVZUUN3ME2zPE46QTF7IN88US=> MkDEV2FPT0WU
MZ7-mel NFz2XXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTN7LkGxPVkh|ryP Mk\lV2FPT0WU
COLO-679 NED1WJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjOR284UUN3ME2zPU46PzB6IN88US=> M3\aOXNCVkeHUh?=
SW872 M3qxcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLGNVdKSzVyPUSwMlIxOzFizszN NGD5NIRUSU6JRWK=
HT-1197 MkPqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTRyLkK4Olkh|ryP MX7TRW5ITVJ?
ES6 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUXkV2JJUUN3ME20NE41PTV{IN88US=> NX:3WGlNW0GQR1XS
SNU-387 Ml;sS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrvTWM2OD12MD64NlM5KM7:TR?= MX\TRW5ITVJ?
T47D NVHTeYVtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvvUWpKSzVyPUSxMlAyQTJizszN MljTV2FPT0WU
G-402 NUjsRVNOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTRzLkC4NFkh|ryP MYjTRW5ITVJ?
MZ1-PC M1K3V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVLJR|UxRTRzLkixJO69VQ>? MoToV2FPT0WU
SW1417 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRTR{LkOwN{DPxE1? NGXwZlBUSU6JRWK=
RERF-LC-MS MnTtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkPSTWM2OD12Mj61NFg4KM7:TR?= MUfTRW5ITVJ?
SF539 MljNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjrNo5KSzVyPUSyMlU2OjlizszN NWS5TIlYW0GQR1XS
ESS-1 Mn25S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTR|LkW1OVgh|ryP NUDMSGNRW0GQR1XS
RO82-W-1 MmTES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml\xTWM2OD12Mz62NVU{KM7:TR?= MXLTRW5ITVJ?
S-117 MoXjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVi3VnNjUUN3ME20N{43QTV6IN88US=> NUPibHBYW0GQR1XS
SW756 NVTLW2JRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjESYFKSzVyPUSzMlgzPDJizszN Ml7MV2FPT0WU
CP66-MEL M3XTZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFnUcphKSzVyPUS1MlcyPDNizszN MmXYV2FPT0WU
H-EMC-SS NEXzVWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3\pWmlEPTB;NEWuO|Y6OyEQvF2= NVHRSHFWW0GQR1XS
NCI-H2052 NXHBT5dvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrNZ4VHUUN3ME20OU46ODJ5IN88US=> NHfSeI5USU6JRWK=
SW684 NWruVXFTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofSTWM2OD12Nz6wNFgyKM7:TR?= MXfTRW5ITVJ?
HCC1569 NFuzN5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NETJU|ZKSzVyPUS3MlE3PThizszN M3HPXXNCVkeHUh?=
KGN MoDjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml62TWM2OD12Nz6zN|EyKM7:TR?= MXjTRW5ITVJ?
LCLC-103H NFHQUpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjBTWM2OD12Nz60OFA1KM7:TR?= MlrjV2FPT0WU
SW780 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXru[IJrUUN3ME20O{43OzVzIN88US=> MlPkV2FPT0WU
NCI-H1703 MoXYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3fnWmlEPTB;NEiuN|M2OiEQvF2= Mom0V2FPT0WU
HCC1395 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWnldpNOUUN3ME20PE44PDN{IN88US=> M{TkWXNCVkeHUh?=

... Click to View More Cell Line Experimental Data

体内研究 Gefitinib 作用于无胸腺裸鼠,抑制SKOV3 或MDA-MB-231 肿瘤生长。Gefitinib高效作用于两种类型肿瘤。处理14天后,MDA-MB-231肿瘤和SKOV3肿瘤生长抑制分别为71%和32%。

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:[1]
+ 展开
  • Cell lines: NR6, NR6M 和 NR6W 细胞
  • Concentrations: 0 μM -2 μM
  • Incubation Time: 72 小时
  • Method: 呈指数生长的细胞,包括 NR6, NR6M, NR6M和NR6W细胞按6倍接种在96孔板上,每孔为2000个细胞, 粘附,随后在PBS 中清洗,然后在含0.5% FCS的培养基上温育过夜。使用不同浓度(0-2 μM) Gefitinib或溶质对照DMSO 和EGF 处理细胞。诱导NR6wtEGFR和NR6W 细胞增殖的EGF最佳浓度已经测定,因此NR6wtEGFR和 NR6W 细胞中分别加入10 nM和 0.1 nM EGF。NR6 和NR6M细胞中不加入EGF。72 小时后,通过MTT 增殖检测测量细胞数。
    (Only for Reference)
动物实验:[3]
+ 展开
  • Animal Models: 携带 SKOV3或MDA-MB-231肿瘤的无胸腺裸鼠
  • Formulation: 0.2% DMSO
  • Dosages: 75 mg/kg
  • Administration: 饲喂处理
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用:
1% CMC Na
30mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 446.90
化学式

C22H24ClFN4O3

CAS号 184475-35-2
稳定性 powder
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • 回答:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

EGFR Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID