Gefitinib (ZD1839)

目录号:S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD1839)是一种EGFR抑制剂,作用于NR6wtEGFR和NR6W细胞中的Tyr1173,Tyr992,Tyr1173和Tyr992,IC50 分别为37 nM,37nM,26 nM和57 nM。

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客户购买Selleck的此次产品后发表的文献93篇:

客户使用该产品的14个实验数据:

  • c, HCC4006 lung cancer cells that have acquired a high-mesenchymal state concomitant with becoming resistant to gefitinib (red curve) exhibit increased sensitivity to GPX4 inhibition.

    Nature, 2017, 547(7664):453-457. Gefitinib (ZD1839) purchased from Selleck.

    Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

  • Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

    (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

  • LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of 5-HT-induced 5-HT3A and 5-HT3AB receptor currents.

    Eur J Pharmacol, 2017, 821:21-28. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

产品安全说明书

EGFR抑制剂选择性比较

生物活性

产品描述 Gefitinib (ZD1839)是一种EGFR抑制剂,作用于NR6wtEGFR和NR6W细胞中的Tyr1173,Tyr992,Tyr1173和Tyr992,IC50 分别为37 nM,37nM,26 nM和57 nM。
特性 Gefitinib是有效的EGFR酪氨酸激酶抑制剂。
靶点
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
体外研究

Gefitinib 作用于高和低表达EGFR的细胞系,包括NR6, NR6M 和 NR6W 细胞系,有效抑制EGFR上全部酪氨酸磷酸化位点。磷酸化位点 Tyr1173和Tyr992敏感性较低,需要高浓度Gefitinib才能抑制。Gefitinib作用于NR6W细胞,高效阻断PLC-γ磷酸化, IC50为27nM。NR6wtEGFR 和NR6M细胞系具有低水平PLC-γ磷酸化, IC50 分别为 43 nM和 369 nM。Gefitinib抑制AKT磷酸化,作用于低EGFR和EGFRvIII表达的细胞系, IC50 分别为220 和263nM。Gefitinib按 0.1 到 0.5μM剂量处理,显著促进而不是废除 NR6M细胞形成集落。然而, 2μM Gefitinib 完全抑制 NR6M 集落形成。Gefitinib 作用于高和低EGFR表达的细胞系,EGF 刺激72小时后,快速抑制EGFR 和ERK 磷酸化,这种作用存在剂量依赖性。[1]Gefitinib抑制 EGF驱动的未转化MCF10A 细胞生长,IC50为 20 nM。[2] Gefitinib 作用于MDA-MB-231细胞,抑制EGF信号。Gefitinib严重抑制细胞数的增多,这种作用存在剂量依赖性。 [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB M{X6e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTBwMEW0JO69VQ>? NYXTUpdtOTZ3MU[0O|M>
NIH3T3 MofwSpVv[3Srb36gRZN{[Xl? NYmxfGd3OC5{IN88US=> NFLESHIzKGh? M1Hr[WROW09? MkLSTY5pcWKrdHnvckBw\iCHR1[gd5RqdXWuYYTl[EBpfW2jbjDldoJDOSCjdYTvdIhwe3Cqb4L5cIF1cW:wIIfpeIghUUN3MDDv[kAxNjBzNESg{txO NHLuNVcyPjR6MEK4OC=>
COS7 Mm\5SpVv[3Srb36gRZN{[Xl? Mnf1PVYh|ryP NXzOSGNqUW6qaXLpeIlwdiCxZjDoeY1idiCMQVuyJHY3OTeIIH31eIFvfCCneIDy[ZN{\WR? MXOxO|E4QDd{Mh?=
A549 NWjoc3lGS3m2b4TvfIlkKEG|c3H5 NIX2fGQ6PiCq M4fhWWROW09? M4PlO2lEPTB;MUOuOVkh|ryP MlH5NVg{OTN6MEe=
K562 NIXtboxEgXSxdH;4bYMhSXO|YYm= NHTt[Y06PiCq M3jsS2ROW09? NFHTdFVKSzVyPUmuN|Yh|ryP M4LD[FE5OzF|OEC3
MDA-MB-231 MUfDfZRwfG:6aXOgRZN{[Xl? NFzqOVI6PiCq MlLHSG1UVw>? MWDJR|UxRTN4Lk[xJO69VQ>? M3vW[VE5OzF|OEC3
MOLT4 MnfYR5l1d3SxeHnjJGF{e2G7 M3HhZVk3KGh? MX7EUXNQ NHfNOFFKSzVyPUG1MlAzKM7:TR?= M3vsXFE5OzF|OEC3
MCF7 M4PacmN6fG:2b4jpZ{BCe3OjeR?= MV:1NEDPxE1? NF;5Znc4OiCq M1T1XGlEPTB;MUKuNFUh|ryP MYexPFc4OThzOR?=
BT474 MmPIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX3VcopZPzJiaB?= MYrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEKWNEe0JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWUQnKyJJdqfGhiRVO1NEBw\iByLkOg{txO NHe2[ZIyQTB{OESyOC=>
HN5 M3HxS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlHMO|IhcA>? M3THTmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSF61JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKge4l1cCCHQ{WwJI9nKDBwMEGg{txO MVixPVAzQDR{NB?=
BA/F3 MV\DfZRwfG:6aXOgRZN{[Xl? NWHoSllLPzJiaB?= NYLHdFFUS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhSkFxRkOgZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKEx6NUjSJI12fGGwdDD3bZRpKEmFNUCgc4YhOC5yMUC4JO69VQ>? MWWxPVI{QTJ{OR?=
NIH3T3 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHr4N3g4OiCq NVnxc5p{T3Kxd4ToJIlvcGmkaYTpc44hd2ZibX;1d4UhVkmKL{PUN{Bk\WyuczDlfJBz\XO|aX7nJIVzSjJiZ3Xu[UB4cXSqIFnDOVAhd2ZiMD61OEDPxE1? MYSxPVY3PTN5Nx?=
MCF7 MX MUXGeY5kfGmxbjDBd5NigQ>? M{HnW|MxKG2rbh?= MmjBTY5pcWKrdHnvckBw\iCEQ2LQJIV5eHKnc4Pl[EB4cXSqIFnDOVAhd2ZiMT6xJO69VQ>? MX:xPVk{Ojl4MB?=
A431 MlPLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWOxNEDPxE1? NUW4Z4RTPzJiaB?= MorwS2k2OD1yLkWxOEDPxE1? M3ezR|E6QTZ7NE[1
A431 M33QbmtqdmG|ZTDBd5NigQ>? MmeyNE4yKM7:TR?= MXGxJIg> NETHO25KdmirYnn0bY9vKG:oIFXHSk1qdmS3Y3XkJGVITlJicHjvd5Bpd3K7bHH0bY9v M1nGbFE6QTZ7NE[1
A431 NIPjTVJMcW6jc3WgRZN{[Xl? NF3DdW4yODBizszN NHHJWoMyOCCvaX6= M4DmRnBzd2yxbnfl[EBqdmirYnn0c5J6KGGldHn2bZR6KG:oIFXHSk1qdmS3Y3XkJGVITlJicHjvd5Bpd3K7bHH0bY9v MnriNVk6Pjl2NkW=
Sf9 MYPLbY5ie2ViQYPzZZk> MljZNVAhdWmw MWHEUXNQ NIPEUYFKdmirYnn0bY9vKG:oIFXHSnIh\XiycnXzd4VlKGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhemWlZYD0c5Ih[XW2b4Doc5NxcG:{eXzheIlwdiC5aYToJGlEPTBib3[gNE4xOzNizszN MWqyNFA2PjR{NR?=
NCI-H1975 NV3Qb5M3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUi1JO69VQ>? MkHvO|IhcA>? Mmn5TWM2OD16LkK2JO69VQ>? MkPRNlAyPTF4N{C=
NCI-H1975 NFvJW2FMcW6jc3WgRZN{[Xl? MlTJNVAh|ryP M2f2VmlvcGmkaYTpc44hd2ZiRVfGVkBifXSxcHjvd5Bpd3K7bHH0bY9v MUiyNFE2OTZ5MB?=
NCI-H1975 MYrGeY5kfGmxbjDBd5NigQ>? NHHPSJEyOCEQvF2= NUPjVohvUW6qaXLpeIlwdiCxZjDFVmJDOiCjdYTvdIhwe3Cqb4L5cIF1cW:w MkDQNlAyPTF4N{C=
NCI-H1975 NVOwVXVQTnWwY4Tpc44hSXO|YYm= M{L5eVUh|ryP MW[0PEBp MoLKTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCjc4Pld5Nm\CCjczDhZ5RqfmG2aX;uJI9nKGOjc4Dhd4UuOw>? M13yVFIxOTVzNkew
A375 NG\EOmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELPfnA1QCCq MXLJR|UxRTJyLkW1JO69VQ>? NE\VU4kzODNyNEWzOy=>
SMMC7721 NFO2N|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInnNIM1QCCq MV3JR|UxRTN|LkGyJO69VQ>? M1T0[lIxOzB2NUO3
DU145 NV7DTWtxS3m2b4TvfIlkKEG|c3H5 MXO1JIQ> MUPEUXNQ MmLQTWM2OD12Mj6xO{DPxE1? NW\3[FlzOjB2Nk[1OVU>
MIAPaCa2 NYnlZ4prS3m2b4TvfIlkKEG|c3H5 MkD5OUBl MkLXSG1UVw>? NEHCdodKSzVyPUWwMlYzKM7:TR?= MUOyNFQ3PjV3NR?=
PANC1 NGDGfIlEgXSxdH;4bYMhSXO|YYm= Mm\qOUBl MlvNSG1UVw>? M4XtNmlEPTB;NECuOFIh|ryP Mn;JNlA1PjZ3NUW=
PC9 NWfQfVAxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWiyZ|dxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBGT0[ULXTl[olkcWWwdDDoeY1idiCSQ{mgZ4VtdHNid3n0bEBGSzVyIH;mJFAvODF5IN88US=> M2TOeFIyOjB6OECy
PC9 NHnLbmNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI\6ZotCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIdzd3e2aD3y[ZNqe3SjboSgbJVu[W5iUFO5JINmdGy|IHX4dJJme3OrbnegSWdHWiCHN{S2Y2E4PTBxVEe5NG0hdXW2YX70JJdqfGhiRVO1NEBw\iB5IN88US=> MXSyNVIxQDhyMh?=
PC3 NXHUS45WT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2LDXFExKM7:TR?= MVy3NkBp M1nBcWROW09? M2PGWmlEPTB;Nz60JO69VQ>? NFjwU3YzOTN3M{W0Oi=>
MDA-MB-468 M2TaS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY[xNFAh|ryP NUfkV5pPPDhiaB?= MWfEUXNQ NVrLcWNVSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJk> MVeyNVQzQTZ|Mh?=
Plasmodium falciparum NIXLPJdyUFSVIIDyc4ZqdGmwZzDmc5IhcW6qaXLpeI9zeyCxZjDQcIF{dW:maYXtJIZidGOrcHHyeY0heHKxbHnm[ZJifGmxbh?= NVXsTWRKOjF6MUewOFU>
NCI-H1993 NXzTOZNTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW[3NkBp MWPEUXNQ NWf0UI1iUUN3ME2wMlEh|ryP NWLm[|RFOjF7MU[0N|M>
NCI-H292 NVvKfZNQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX23NkBp MVPEUXNQ M{\1d2lEPTB;MD6wNkDPxE1? NX\TS49ROjF7MU[0N|M>
A431 NV3EVIRyU2mwYYPlJGF{e2G7 NX;5SJBpOiCq MYrEUXNQ NVLUUmtHUW6qaXLpeIlwdiCxZjDFS2ZTKHCqb4PwbI9zgWyjdHnvckBqdiCHR1[td5RqdXWuYYTl[EBpfW2jbjDBOFMyKHerdHigTWM2OCCxZjCwMlAxOSEQvF2= MojrNlIyODFzM{K=
MCF7 NX7mPGU1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmewNVAxyqEQvHevcWw> M3m1VlQ5KGh? NXrZeIU{TE2VTx?= MljzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKg[4Vv\SC5aYToJGlEPTBib3[gNVIvODVizszN NWjLO5p7OjJzMUmxN|A>
BT474 MkDWR5l1d3SxeHnjJGF{e2G7 NUnYXFVvOTBizszN M4XwWlQ5KGh? NXHOTFAzS3m2b4TvfIlkcXS7IHHnZYlve3Ric3XyeY0ue3Sjco\l[EBpfW2jbjDCWFQ4PCClZXzsdy=> NWqyZ|Z3OjJzM{izNFg>
MCF7 NIPvVXdEgXSxdH;4bYMhSXO|YYm= MYOxNEDPxE1? M2PNc|Q5KGh? NH3wfJZEgXSxdH;4bYNqfHliYXfhbY5{fCC|ZYL1cU1{fGG{dnXkJIh2dWGwIF3DSlch[2WubIOgc5Zmei2neIDy[ZN{cW6pIFXyZmIzKGOnbHzz MlLxNlIyOzh|MEi=
HCC827 MV\LbY5ie2ViQYPzZZk> NIrYN|ExNjF{NTFOwG0> M3zNc|I1KGh? NVXQc3A1TE2VTx?= NU\vfWd4UW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62IGmxNFY5KGG3dH;wbI9{eGixconsZZRqd25? MWmyNlM{QTN2Mh?=
HCC827 MWrLbY5ie2ViQYPzZZk> MlntNE4yOjVizszN M4LI[|I1KGh? MofOSG1UVw>? MoTqTY5pcWKrdHnvckBw\iCHR1\SJIRmdCCHN{S2MWE4PTBibYX0ZY51NW2nZHnheIVlKEW{azDwbI9{eGixconsZZRqd25? NHPre3IzOjN|OUO0Ni=>
HCC827 NHvMVI5McW6jc3WgRZN{[Xl? MmL4NE4yOjVizszN MkXCNlQhcA>? MW\EUXNQ MnLaTY5pcWKrdHnvckBw\iCHR1\SJIRmdCCHN{S2MWE4PTBibYX0ZY51NW2nZHnheIVlKEGtdDDwbI9{eGixconsZZRqd25? M3vYPFIzOzN7M{Sy
A431 Moi2T4lv[XOnIFHzd4F6 MnrmNVAxKM7:TR?= MXKzNEBucW5? NHj5VFZKdmirYnn0bY9vKG:oIFXHSnIh\GW{aY\l[EBnem:vIHj1cYFvKEF2M{GgZ4VtdCCvZX3idoFv\SCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHCxbImtS4x2NVS7cjDwbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFEvODhizszN NXPuN3NzOjJ4OUi3PFI>
H460 NEjLU4hEgXSxdH;4bYMhSXO|YYm= MVS3NkBp NIO5TXhKSzVyPUWuOVkh|ryP NHXEcVAzOjd{N{S0PS=>
HT-29 NUDLUVl2S3m2b4TvfIlkKEG|c3H5 MYC3NkBp MoHhTWM2OD1|LkO2JO69VQ>? NUTIcJM2OjJ5Mke0OFk>
SGC7901 MV3DfZRwfG:6aXOgRZN{[Xl? NWPBblFtPzJiaB?= NIPue4pKSzVyPUGwMlI3KM7:TR?= M4HS[VIzPzJ5NES5
A498 NEniV4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFi2dIRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG0PVgh[2WubIOg[ZhxemW|c3nu[{BGT0[UIHHu[EBUWkNid3n0bEBKSzVyIH;mJFgvPiEQvF2= MYSyNlgyQDh2OB?=
Calu3 NFi1NnlEgXSxdH;4bYMhSXO|YYm= NVTt[Xg{OTBizsznM41N NETMd5Y4OiCq MU\JR|UxRTFwNjFOwG0> MV:yN|EyPjF4OB?=
FADU MkPjR5l1d3SxeHnjJGF{e2G7 NYXRZ4I{OTBizsznM41N NUjhR4ZXPzJiaB?= MoK5TWM2OD1yLkC1JO69VQ>? M{TCdVI{OTF4MU[4
HCC827 MXLDfZRwfG:6aXOgRZN{[Xl? NWrVOWE5OTBizsznM41N MVq3NkBp MXHJR|UxRTBwMECxOUDPxE1? M1ywNVI{OTF4MU[4
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HCC827 MojkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LwOlczKGh? M2G4eGlEPTB;MD6wNVIh|ryP NH;RNIwzOzl5M{G2PC=>
A549 MnLvT4lv[XOnIFHzd4F6 M1;pVFEh|ryP MYKxJIg> MWjEUXNQ NH7iSpVKdmirYnn0bY9vKG:oIFXHSnIh[XW2b4Doc5NxcG:{eXzheIlwdiC5aYToJGlEPTBib3[gNE4xOzl7IN88US=> M3:zVlI{QTh6M{W0
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BCRP-MDCK2 MoLESpVv[3Srb36gRZN{[Xl? NXjtc|VHOS56IN88US=> M1PiUlczKGh? M3PzVGROW09? M2[2[2lv[3KnYYPlJI9nKHCuYYPtZUBu\W2kcnHu[UBmgHC{ZYPzbY9vKG:oIFLDVnAh\XiycnXzd4lv\yC{ZYDvdpRmeiCpZX7lJGdHWA>? NH;0PGkzPDF6NEKxNy=>
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NCI-H1975 NYn1bVNETnWwY4Tpc44hSXO|YYm= Mn7TNVAxKM7:TR?= MVOxOVAhdWmw NXvxbZpzTE2VTx?= NGrHRnBKdmS3Y4Tpc44hd2ZiTl:gdJJw\HWldHnvckBqdiCqdX3hckBPS0lvSEG5O|Uh[2WubIOgbIFz[m:{aX7nJGVITlJiTEi1PHIwXDd7MF2gcZV1[W62 NVqyVHhwOjR4MEe1PVE>
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NCI-H1975 Mn76SpVv[3Srb36gRZN{[Xl? NGnuR2UyOCEQvF2= MV64JIg> M2HGNGROW09? NVm4WFc2\G:nczDuc5QhcW6qaXLpeIkhTUeIUjDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iRWLLJJBpd3OyaH;yfYxifGmxbh?= NXi0U4VIOjNzMU[xOlg>
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BT-20 MlHtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITVT5RKSzVyPUKuNlY4PDRizszN NHLISY1USU6JRWK=
A498 MkXSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVq2NFRVUUN3ME2yMlI6PDF3IN88US=> M1\OfHNCVkeHUh?=
IGROV-1 NYjIeXdjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUTJR|UxRTJwM{GyN|ch|ryP M{PJWXNCVkeHUh?=
JVM-3 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH7SfIdKSzVyPUKuN|Q3PDNizszN NVzL[YpPW0GQR1XS
NCI-H2170 M13PVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkHWTWM2OD1{LkO0O|g{KM7:TR?= MlPMV2FPT0WU
EW-18 M3\5b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXERmFKSzVyPUKuN|U1PzFizszN M1v4UXNCVkeHUh?=
KU812 NUPWfpJ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjQTWM2OD1{LkO2O|c6KM7:TR?= Ml\4V2FPT0WU
NY MnnNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTJwM{eyO{DPxE1? NYLDcYNRW0GQR1XS
HTC-C3 NVPHXlV7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTJwNEKyOlch|ryP MWnTRW5ITVJ?
CFPAC-1 NGHDZ3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TPRWlEPTB;Mj60N|g3PCEQvF2= NET2T3lUSU6JRWK=
HH NH[yZY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTJwNEm4OVQh|ryP M4rBWXNCVkeHUh?=
MKN7 MnO5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTJwNkK3OFgh|ryP M2DEcHNCVkeHUh?=
TE-8 NH[x[JVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2G3XWlEPTB;Mj62N|Y2OyEQvF2= MlrjV2FPT0WU
MC-IXC M3zlWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoL5TWM2OD1{Lk[0OFU{KM7:TR?= MWnTRW5ITVJ?
DMS-79 NYT4eVV6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXG1UoV2UUN3ME2yMlY6Ojh2IN88US=> NUjVbnN3W0GQR1XS
Detroit562 NG\jR|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlj4TWM2OD1{Lk[5PFQ2KM7:TR?= NF31W2VUSU6JRWK=
HuP-T4 M4H3UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFG2e2JKSzVyPUKuO|I5OThizszN MVfTRW5ITVJ?
QIMR-WIL MlK3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjKVpJKSzVyPUKuO|U6PTJizszN NFvad5VUSU6JRWK=
BB65-RCC M4[1Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXT6OWN{UUN3ME2yMlgxPzF|IN88US=> MmHMV2FPT0WU
PC-3 NILRboJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTJwOEOzNVch|ryP NXzVbIpEW0GQR1XS
OVCAR-3 NUTzPFIzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV7JR|UxRTJwOEW1PVQh|ryP Mmi2V2FPT0WU
KYSE-510 M3Pifmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoW2TWM2OD1{Lki2O|g3KM7:TR?= Mm\mV2FPT0WU
HC-1 M3mxXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYfJR|UxRTJwOUSwNVIh|ryP MkXMV2FPT0WU
NCI-H1693 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3fPeGlEPTB;Mj65OVg6PyEQvF2= NIrmPI1USU6JRWK=
DU-4475 NVrMZnZ7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2LmTGlEPTB;Mj65PVA2OiEQvF2= MXzTRW5ITVJ?
COLO-680N M2TRSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmfhTWM2OD1|LkCwOFk2KM7:TR?= NWT1W|JWW0GQR1XS
MN-60 NHizb4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nHZ2lEPTB;Mz6wOFk4PCEQvF2= M2PEbnNCVkeHUh?=
BCPAP MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGize3JKSzVyPUOuNFgzQTVizszN MW\TRW5ITVJ?
647-V NFfQSVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnnFTWM2OD1|LkG2NlIh|ryP NX;SbGVjW0GQR1XS
HT-1376 MoPOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTNwMkC1NFYh|ryP MVrTRW5ITVJ?
NCI-H1793 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXfHd5BXUUN3ME2zMlIyODl6IN88US=> NYfOeGZ2W0GQR1XS
SW1463 NH;tTm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml7NTWM2OD1|LkK2NVkzKM7:TR?= MnrRV2FPT0WU
NCI-H727 MnLIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\BTWM2OD1|LkO4OVg1KM7:TR?= Mki4V2FPT0WU
LB771-HNC MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zHN2lEPTB;Mz6zPVEzPiEQvF2= NF3J[FBUSU6JRWK=
D-542MG NXK1eFJqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFPoWW5KSzVyPUOuOFQzOjJizszN MX\TRW5ITVJ?
NTERA-S-cl-D1 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTNwNES5OVch|ryP NX7mS2VlW0GQR1XS
C8166 MlnOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFPJNW1KSzVyPUOuOFY2OjFizszN Mn7XV2FPT0WU
639-V MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mon2TWM2OD1|LkW3O|kzKM7:TR?= MXnTRW5ITVJ?
TE-1 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTKTWM2OD1|LkW5NVY6KM7:TR?= NY\vRlJuW0GQR1XS
OAW-28 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDDTWM2OD1|LkW5OFI3KM7:TR?= NEm0ZYJUSU6JRWK=
SK-LU-1 NFHm[lBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{iyZ2lEPTB;Mz62NFY1PSEQvF2= MnzBV2FPT0WU
CaR-1 NGLiTpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33nPGlEPTB;Mz62NVA1QCEQvF2= NX7lPWtFW0GQR1XS
A2780 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnWzTWM2OD1|Lk[0NFg6KM7:TR?= MW\TRW5ITVJ?
C2BBe1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTNwNkS0NFch|ryP NX[5RphtW0GQR1XS
SK-MES-1 M3XxZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2DOUmlEPTB;Mz62OFYyPCEQvF2= MmrUV2FPT0WU
PANC-10-05 MlTyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTNwN{KwO|Ih|ryP MV7TRW5ITVJ?
J-RT3-T3-5 MoPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHleI94UUN3ME2zMlc2PDB4IN88US=> MlHlV2FPT0WU
ES4 M3PQZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rDe2lEPTB;Mz64NlI5PiEQvF2= NG\FOpZUSU6JRWK=
NCI-H28 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnn5TWM2OD1|LkiyPVYyKM7:TR?= NV\qPYN6W0GQR1XS
NCI-H1155 M1HzUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXhSGU2UUN3ME2zMlg1OTV7IN88US=> NFixT5ZUSU6JRWK=
KURAMOCHI M3XyTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml:2TWM2OD1|Lki2NFU3KM7:TR?= NFTBOnJUSU6JRWK=
NCI-H69 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjZTWM2OD1|LkmwNVc5KM7:TR?= Mlz3V2FPT0WU
IST-MES1 M4P5V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\DTWM2OD1|Lkm0NFI6KM7:TR?= NG\1PXFUSU6JRWK=
NH-12 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDNNWJ[UUN3ME2zMlk1OTh5IN88US=> NX7k[WJGW0GQR1XS
JVM-2 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIX6R|FKSzVyPUOuPVQ4PTJizszN MXXTRW5ITVJ?
EW-16 NEDqVmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MofGTWM2OD1|Lkm1OVc4KM7:TR?= M1ywT3NCVkeHUh?=
NCI-H441 NGW5VIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M170NGlEPTB;Mz65O|AyPCEQvF2= NHPBOmJUSU6JRWK=
RCC10RGB MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NI\OTVRKSzVyPUSuNFEzPDZizszN M4jWeXNCVkeHUh?=
TCCSUP M1vFPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkK5TWM2OD12LkGxNFUh|ryP M{nHR3NCVkeHUh?=
HL-60 MmPHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\tZYRKSzVyPUSuNVIzPDFizszN MWrTRW5ITVJ?
EW-11 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVS5XmNuUUN3ME20MlE{OjZzIN88US=> M1LiOHNCVkeHUh?=
KARPAS-299 NHLUflJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXIcYlTUUN3ME20MlMyODF|IN88US=> NX\vZol[W0GQR1XS
SNU-C2B NWTXbVJpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIjMemNKSzVyPUSuN|Q3QDZizszN MmHuV2FPT0WU
LU-139 M3SxUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVP1[o1jUUN3ME20MlM4OjR5IN88US=> M1XPNnNCVkeHUh?=
HCC1937 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrUTWM2OD12LkO3PVk{KM7:TR?= NXnmTYk2W0GQR1XS
GR-ST NWSxSo5qT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3uzeWlEPTB;ND60N|E5KM7:TR?= M4jSUnNCVkeHUh?=
SK-OV-3 M1PJTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFL2TWtKSzVyPUSuOFYxPDJizszN Moq0V2FPT0WU
A172 M4rVVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\tTWM2OD12LkW1OVIyKM7:TR?= Mn3LV2FPT0WU
NB10 M2jRNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTRwNUm3PFEh|ryP MlLWV2FPT0WU
P30-OHK MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnoeJhvUUN3ME20MlgyQTJ7IN88US=> M2\ZbHNCVkeHUh?=
KYSE-520 NW[2WVh6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTRwOEiwNVgh|ryP Mlm0V2FPT0WU
KNS-62 M3foNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWnafIFpUUN3ME20MlkxQDB5IN88US=> NUm0cYFPW0GQR1XS
ES1 NYj5RZFZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml7UTWM2OD12Lkm2NFM2KM7:TR?= M1rwVnNCVkeHUh?=
KOSC-2 NFzH[oxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTRwOUm5OVkh|ryP MmqwV2FPT0WU
TE-9 M4S5TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3KxR2lEPTB;NT6wNVUzPSEQvF2= MWDTRW5ITVJ?
NCI-H1838 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17H[2lEPTB;NT6wNlA4PiEQvF2= NXOx[HpnW0GQR1XS
KP-N-YN NVrFcmlqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEGybHpKSzVyPUWuNVI2QTFizszN NFPkV4hUSU6JRWK=
ALL-PO NF3Ifm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfXTWM2OD13LkKyOVg4KM7:TR?= MYHTRW5ITVJ?
NOMO-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2TYS2lEPTB;NT6yN|E4PiEQvF2= NIXCNZNUSU6JRWK=
CGTH-W-1 NVrUNlRNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYX6TG5kUUN3ME21MlM5PTh2IN88US=> NF\pV4pUSU6JRWK=
ATN-1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWT1bGZpUUN3ME21MlQyOTh2IN88US=> MUHTRW5ITVJ?
AGS MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLsTWM2OD13LkS5OlE5KM7:TR?= NHSxNWtUSU6JRWK=
SW13 M2n0d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJR|UxRTVwNUOyOlUh|ryP MnjlV2FPT0WU
SW1990 M2\oVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1zqWmlEPTB;NT62NlI5PSEQvF2= MVPTRW5ITVJ?
BFTC-905 MlXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn6yTWM2OD13Lk[1PFc5KM7:TR?= NVjHbZhIW0GQR1XS
RMG-I MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmfaTWM2OD13Lk[1PVQzKM7:TR?= MUXTRW5ITVJ?
HD-MY-Z NXPtOFFWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PLNWlEPTB;NT62PFU5OSEQvF2= NHW0eFVUSU6JRWK=
HCC1187 NYjZ[FN6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7Pe|Q2UUN3ME21MlcyODN{IN88US=> MXjTRW5ITVJ?
HCC2998 NID4eVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DGO2lEPTB;NT63NVg4PyEQvF2= MV3TRW5ITVJ?
RT-112 Mo\MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfpVXJMUUN3ME21MlkxOjR3IN88US=> Ml[wV2FPT0WU
CAKI-1 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTZwMEC5PFUh|ryP M2jrN3NCVkeHUh?=
HDLM-2 NI\1OYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4iyTmlEPTB;Nj6wNVQ5QSEQvF2= NIjreoVUSU6JRWK=
TE-10 Mo\KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnzBTWM2OD14LkC0PFM{KM7:TR?= MVHTRW5ITVJ?
KE-37 NX;FUWVvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M323[2lEPTB;Nj6wO|I2QCEQvF2= NYnx[FhuW0GQR1XS
RCM-1 MnPtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2KzPGlEPTB;Nj6wO|cyQSEQvF2= NXzmUZlvW0GQR1XS
Mewo NYH2VpRWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmPxTWM2OD14LkGxOFg4KM7:TR?= MmPhV2FPT0WU
L-428 MnTTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkDjTWM2OD14LkG4NFM4KM7:TR?= NIC1XZZUSU6JRWK=
8-MG-BA NWXpS5FST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;rTWM2OD14LkG5N|c6KM7:TR?= NFj0WFdUSU6JRWK=
SK-MEL-28 NIexZY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTZwMkC5Olch|ryP M2DFXXNCVkeHUh?=
VA-ES-BJ NInvPJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTZwM{C0OlIh|ryP MXXTRW5ITVJ?
CAL-120 NVfUSVRGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH74XVNKSzVyPU[uOFUyPjJizszN NEPGfYdUSU6JRWK=
GAMG NUfsRYl1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFPHOWNKSzVyPU[uOVA3OiEQvF2= MmfHV2FPT0WU
MSTO-221H NYPP[ZcxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXTmNFVsUUN3ME22MlUyOTdizszN MoDXV2FPT0WU
GCT MontS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYLYfJJRUUN3ME22MlU{Ozh5IN88US=> NIPJe2xUSU6JRWK=
SH-4 NF36W4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHj2RW5KSzVyPU[uOVg5PDRizszN Mo[0V2FPT0WU
Calu-3 MkjjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGf5UWNKSzVyPU[uOlU2PjhizszN NUfubWllW0GQR1XS
SK-HEP-1 NH7VXJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXuzPWhZUUN3ME22MlcyPzV|IN88US=> M{\2ZnNCVkeHUh?=
PFSK-1 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXKTWM2OD14LkezOFc5KM7:TR?= MkTVV2FPT0WU
NB12 M{\VRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGCxXHZKSzVyPU[uO|g1ODRizszN NIfqPVFUSU6JRWK=
A388 NYmxfHZ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvINnlKSzVyPU[uPFk2OiEQvF2= NWD0NoFLW0GQR1XS
KLE NH7GXYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTZwOU[1NVYh|ryP MYLTRW5ITVJ?
TE-11 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTdwMES5Olch|ryP M4D3W3NCVkeHUh?=
NCI-H2405 M4jHVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\1TWM2OD15LkC4N|k{KM7:TR?= MYrTRW5ITVJ?
OVCAR-4 MoDKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\5TWM2OD15LkG1N|Uh|ryP MYHTRW5ITVJ?
KP-N-YS MmjUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFu2[Y9KSzVyPUeuNVgyPCEQvF2= MlnPV2FPT0WU
NOS-1 M4fSeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvYdHNKUUN3ME23MlI2QDJ6IN88US=> MX3TRW5ITVJ?
DMS-114 NH31TppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MonuTWM2OD15LkK5OlMyKM7:TR?= MVHTRW5ITVJ?
NCI-H2030 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVrZNGlTUUN3ME23MlM{Pjl6IN88US=> NFfyZotUSU6JRWK=
HEL NYT4OYlKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1Xa[mlEPTB;Nz6zO|UzOSEQvF2= NGDiUJBUSU6JRWK=
CAL-62 NHrtdYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlv0TWM2OD15LkO5PFY3KM7:TR?= NWHxfWc2W0GQR1XS
COLO-668 MoD1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEH6NZRKSzVyPUeuOFM6PjdizszN M3H6XXNCVkeHUh?=
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IGR-1 NHXSd|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfoTWM2OD1|Nj63NFc2KM7:TR?= MnW3V2FPT0WU
SW626 M2jRZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTN5LkC0NVch|ryP NYW2OodjW0GQR1XS
ONS-76 NGXvRVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV7JR|UxRTN5LkGzNFkh|ryP MVjTRW5ITVJ?
T98G MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUS5NpNkUUN3ME2zO{45PjN3IN88US=> NF7TS3BUSU6JRWK=
NB14 NX32d2JCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TLbGlEPTB;M{iuOFU1PCEQvF2= MXHTRW5ITVJ?
D-423MG NX7UNJNQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3\IUGlEPTB;M{iuOFg3QSEQvF2= NUTvSlVJW0GQR1XS
NCI-H510A M2m0c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHH0NJFKSzVyPUO4MlY1PTFizszN MV;TRW5ITVJ?
NMC-G1 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\t[mlEPTB;M{iuO|IzOiEQvF2= M2\KdXNCVkeHUh?=
SW948 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXzvUFRQUUN3ME2zPE45QTR7IN88US=> NX22emxXW0GQR1XS
MFM-223 NGn6ephIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTN6Lkm0N|Eh|ryP NEHXWJRUSU6JRWK=
PAN1 M4n2Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVfnOlNDUUN3ME2zPE46QTF7IN88US=> MWfTRW5ITVJ?
MZ7-mel M2S4TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkX5TWM2OD1|OT6xNVk6KM7:TR?= MkjYV2FPT0WU
COLO-679 NUL2b45XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{LtSWlEPTB;M{muPVcxQCEQvF2= MmfRV2FPT0WU
SW872 MmjrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDPe3JKSzVyPUSwMlIxOzFizszN M4XPdnNCVkeHUh?=
HT-1197 M3[4cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF24c3lKSzVyPUSwMlI5PjlizszN MmL4V2FPT0WU
ES6 M2q3Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn[4TWM2OD12MD60OVUzKM7:TR?= NIrI[XZUSU6JRWK=
SNU-387 Ml\BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTRyLkiyN|gh|ryP MVHTRW5ITVJ?
T47D M4Szbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULMV4dVUUN3ME20NU4xOTl{IN88US=> MVXTRW5ITVJ?
G-402 M4PHVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVTBd3RFUUN3ME20NU4xQDB7IN88US=> M4rJ[XNCVkeHUh?=
MZ1-PC NH7tfINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3rlfmlEPTB;NEGuPFEh|ryP NUDDTFJGW0GQR1XS
SW1417 NIX4ZppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWTJR|UxRTR{LkOwN{DPxE1? NGLNXohUSU6JRWK=
RERF-LC-MS NWLRUGN7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2[4eGlEPTB;NEKuOVA5PyEQvF2= MXLTRW5ITVJ?
SF539 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTR{LkW1Nlkh|ryP M4rkd3NCVkeHUh?=
ESS-1 M4G1OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NY\vWYZRUUN3ME20N{42PTV6IN88US=> M4PIN3NCVkeHUh?=
RO82-W-1 NYTKXJBIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTR|Lk[xOVMh|ryP MkDDV2FPT0WU
S-117 M2PrWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzxTWM2OD12Mz62PVU5KM7:TR?= NEXXOolUSU6JRWK=
SW756 NVi1[GdRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{Hqc2lEPTB;NEOuPFI1OiEQvF2= NFPEVnVUSU6JRWK=
CP66-MEL MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWLhcpJmUUN3ME20OU44OTR|IN88US=> M1fuVnNCVkeHUh?=
H-EMC-SS M4XpVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmHQTWM2OD12NT63Olk{KM7:TR?= M323fHNCVkeHUh?=
NCI-H2052 M3vUXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlLvTWM2OD12NT65NFI4KM7:TR?= NXTUR2tXW0GQR1XS
SW684 M3;hR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnftTWM2OD12Nz6wNFgyKM7:TR?= NFHJN5NUSU6JRWK=
HCC1569 NIG4co5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFfBR2hKSzVyPUS3MlE3PThizszN NYfJc|RnW0GQR1XS
KGN MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vpcGlEPTB;NEeuN|MyOSEQvF2= NIn4boNUSU6JRWK=
LCLC-103H Mne0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XYXGlEPTB;NEeuOFQxPCEQvF2= NV;PfmFIW0GQR1XS
SW780 NYfxVnQzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTR5Lk[zOVEh|ryP NF63dXBUSU6JRWK=
NCI-H1703 NVHlTGZlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MY\JR|UxRTR6LkOzOVIh|ryP NXLPPXA5W0GQR1XS
HCC1395 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrmdZNPUUN3ME20PE44PDN{IN88US=> NUCyeGhMW0GQR1XS

... Click to View More Cell Line Experimental Data

体内研究 Gefitinib 作用于无胸腺裸鼠,抑制SKOV3 或MDA-MB-231 肿瘤生长。Gefitinib高效作用于两种类型肿瘤。处理14天后,MDA-MB-231肿瘤和SKOV3肿瘤生长抑制分别为71%和32%。

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:[1]
+ 展开
  • Cell lines: NR6, NR6M 和 NR6W 细胞
  • Concentrations: 0 μM -2 μM
  • Incubation Time: 72 小时
  • Method: 呈指数生长的细胞,包括 NR6, NR6M, NR6M和NR6W细胞按6倍接种在96孔板上,每孔为2000个细胞, 粘附,随后在PBS 中清洗,然后在含0.5% FCS的培养基上温育过夜。使用不同浓度(0-2 μM) Gefitinib或溶质对照DMSO 和EGF 处理细胞。诱导NR6wtEGFR和NR6W 细胞增殖的EGF最佳浓度已经测定,因此NR6wtEGFR和 NR6W 细胞中分别加入10 nM和 0.1 nM EGF。NR6 和NR6M细胞中不加入EGF。72 小时后,通过MTT 增殖检测测量细胞数。
    (Only for Reference)
动物实验:[3]
+ 展开
  • Animal Models: 携带 SKOV3或MDA-MB-231肿瘤的无胸腺裸鼠
  • Formulation: 0.2% DMSO
  • Dosages: 75 mg/kg
  • Administration: 饲喂处理
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
1% CMC Na
30mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 446.90
化学式

C22H24ClFN4O3

CAS号 184475-35-2
稳定性 powder
in solvent
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00025675 Completed Brain and Central Nervous System Tumors Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins|National Cancer Institute (NCI) October 9 2001 Phase 2
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co. Ltd. September 8 2016 Phase 1
NCT00684385 Completed Non Small Cell Lung Cancer|Cancer of the Head and Neck AstraZeneca August 8 2001 Phase 4
NCT01933347 Active not recruiting Non Small Cell Lung Cancer Guangdong Association of Clinical Trials April 7 2014 Phase 2
NCT02411448 Recruiting Metastatic Non-Small Cell Lung Cancer Eli Lilly and Company May 6 2015 Phase 3
NCT03486496 Not yet recruiting Lung Adenocarcinoma|EGFR Mutation Fujian Cancer Hospital June 5 2018 Phase 2

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • 回答:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID